Prealbumin
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Native human prealbumin, also known as transthyretin, is a homotetrameric serum and cerebrospinal fluid protein which acts as a carrier for the thyroid hormone thyroxine. Each monomer is a 127 amino acid polypeptide. Prealbumin is synthesised principally in the liver and choroid plexus from where it is secreted into the serum and cerebrospinal fluid respectively.
Product Details
- Target Species
- Human
- Product Form
- Purified protein from human serum - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml sterile PBS. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Buffer Solution
- 0.02M Ammonium bicarbonate (NH4HCO3).
- Preservative Stabilisers
- None present
- Purity
- >96%
SDS-PAGE confirms prealbumin as the major component. Under certain conditions dimer and monomer subunits can be seen. Cellulose acetate electrophoresis shows one band only with a higher electrophoretic mobility than albumin. - Approx. Protein Concentrations
- Total protein concentration 1.0 mg/ml after reconstitution.
- Protein Molecular Weight
- 60 kDa (tetramer)
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P02766
- Entrez Gene
- TTR
- GO Terms
- GO:0005576 extracellular region
- GO:0005179 hormone activity
- GO:0005737 cytoplasm
- GO:0006810 transport
- Regulatory
- For research purposes only
Applications of Prealbumin
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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