Prealbumin
Native human prealbumin (7600-0604) used in a protein aggregation experiment.
Image caption:
Validation of the protein aggregation assay.
Representative images for the internalization and accumulation of in vitro generated transthyretin (TTR) aggregates in ADTs. TTR aggregates were generated by incubation of recombinant human TTR in acetate buffer (see “Methods”). ADTs were treated with native TTR or aggregated TTR, fixed at 0 h or overnight after 20-min incubation on ice and then stained with ProteoStat dye (red). The nuclei were stained with DAPI (blue). Bar: 20 μm.
From: Cheng S, Banerjee S, Daiello LA, Nakashima A, Jash S, Huang Z, Drake JD, Ernerudh J, Berg G, Padbury J, Saito S, Ott BR, Sharma S.
Novel blood test for early biomarkers of preeclampsia and Alzheimer's disease.
Sci Rep. 2021 Aug 5;11(1):15934.
doi: 10.1038/s41598-021-95611-5..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Native human prealbumin, also known as transthyretin, is a homotetrameric serum and cerebrospinal fluid protein which acts as a carrier for the thyroid hormone thyroxine. Each monomer is a 127 amino acid polypeptide. Prealbumin is synthesised principally in the liver and choroid plexus from where it is secreted into the serum and cerebrospinal fluid respectively.
Product Details
- Target Species
- Human
- Product Form
- Purified protein from human serum - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml sterile PBS. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Buffer Solution
- 0.02M Ammonium bicarbonate (NH4HCO3).
- Preservative Stabilisers
- None present
- Purity
- >96%
SDS-PAGE confirms prealbumin as the major component. Under certain conditions dimer and monomer subunits can be seen. Cellulose acetate electrophoresis shows one band only with a higher electrophoretic mobility than albumin. - Approx. Protein Concentrations
- Total protein concentration 1.0 mg/ml after reconstitution.
- Protein Molecular Weight
- 60 kDa (tetramer)
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P02766
- Entrez Gene
- TTR
- GO Terms
- GO:0005576 extracellular region
- GO:0005179 hormone activity
- GO:0005737 cytoplasm
- GO:0006810 transport
- Regulatory
- For research purposes only
Applications of Prealbumin
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Prealbumin
-
Clement, C.C. et al. (2016) Autoimmune response to transthyretin in juvenile idiopathic arthritis.
JCI Insight. 1 (2): e85633. -
Cheng, S. et al. (2021) Novel blood test for early biomarkers of preeclampsia and Alzheimer's disease.
Sci Rep. 11 (1): 15934.
Fluorescent Spectraviewer
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