MMP-3

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Recombinant Human MMP-3

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP304
Datasheet
E
SDS
10 µg loader

Recombinant Human MMP-3

Matrix metalloproteinase-3 (MMP-3), also known as stromelysin-1 (SL-1) and transin-1 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells intima and platelets (Cui et al. 2017).

MMP-3 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 57 kDa pro-MMP-3. This is then further cleaved by proteolytic enzymes to create the 45 kDa active form.

Like other MMPs, MMP-3 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-3 degrades the collagen proteins, type II, IV, IX as well as the proteoglycans elastin fibronectin and laminin (Cui et al. 2017). Through the degradation of these ECM components, growth factors and adhesion molecules, MMP-3 can modify the extracellular environment and modulate cell to cell signaling. As such, MMP-3 is implicated in synaptic plasticity through enzymatic modification of ECM constituents (Lech et al. 2019).

This recombinant Human MMP-3 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human MMP-3 antibody, clone I08-9G7 (MCA6258GA) as a capture antibody and biotinylated Mouse anti Human MMP-3 antibody, clone G05-6A10 (MCA6259B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Recombinant protein expressed in E. coli and purified by ion exchange chromatography
Buffer Solution
50 mM Tris-HCl, pH8.0, 0.15 M NaCl
Preservative Stabilisers
1.0% Trehalose
Purity
≥95 % determined by coomassie blue staining of a reducing SDS-PAGE gel
Approx. Protein Concentrations
100 μg/ml after reconstitution
Protein Molecular Weight
21 kDa

Storage Information

Storage
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
P08254
Entrez Gene
MMP3
GO Terms
GO:0005509 calcium ion binding
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
GO:0043231 intracellular membrane-bounded organelle
Regulatory
For research purposes only

Applications of MMP-3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human MMP-3 MCA6258GA E 0.1 mg loader
Mouse anti Human MMP-3:Biotin MCA6259B E 0.1 mg loader

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