Leptin

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Recombinant Human Leptin

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP198 E FN WB 1 mg
Recombinant human leptinE. coli. Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.

Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum abumin (BSA) is recommended.
Preparation
Purified recombinant leptin expressed in E.coli. Sterile filtered through a 0.2 micron filter.
Buffer Solution
Lyophilized with 0.1% TFA
Preservative Stabilisers
None present
Activity
PHP198 is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
Purity
>98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Molecular Weight
16.0kD (147 amino acid residues).
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P41159 Related reagents
Entrez Gene
LEP Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006112 energy reserve metabolic process
GO:0008343 adult feeding behavior
GO:0032099 negative regulation of appetite
Regulatory
For research purposes only

Applications of Leptin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human leptin may be use as a standard for ELISA applications with purified human leptin antibody (AHP1616).
Western Blotting
Recombinant human leptin may be used as the positive control for Western Blotting applications with a purified human leptin antibody (AHP1616).