Laminin

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Native Human Laminin

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5620-0604 E FN 0.1 mg
Native Human Laminin is purified protein from human placenta and supplied as a liquid.

Laminin is an endothelial adhesion glycoprotein composed of 3 chains designated A (MW ~400 kDa), B1 (MW ~210 kDa) and B2 (MW ~200 kDa) held together by disulphide bonds in cruciform shape.

Merck Index: 12: 5364
CAS number: 114956-81-9
Bio-Rad Antibodies recommend to thaw this solution slowly at 2-8°C to avoid gel formation.

Product Details

Target Species
Human
Preparation
Purified by immunoaffinity from pepsinized human placenta.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
None present
Purity
SDS PAGE: >90%
Approx. Protein Concentrations
0.5 mg/ml
Molecular Weight
In electrophoresis a major band is observed at 60 kDa and a minor band at 170-190 kDa.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Laminin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Instructions For Use
Care must be taken when using at room temperature. It is advisable to use the product as soon as possible. If a gel forms then it is not possible to reactivate for use.

Product Specific References

References for Laminin

  1. Koeppen, A.H. et al. (2016) Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation.
    Acta Neuropathol Commun. 4 (1): 46.