IL-7
Recombinant Human Interleukin-7
- Product Type
- Recombinant Protein
- Specificity
- IL-7
Antibody Quality and Performance Guarantee
| Recombinant human IL-7 is a 17.4 kDa protein comprised of 153 amino acids corresponing to the ful-length mature sequence. IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells. In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of > 2 x 106 units/mg. Recombinant human IL-7 is recognized by Rat anti Human IL-7 antibody clone BVD10-40F6 in ELISA. |
- Target Species
- Human
- Product Form
- Purified recombinant protein expressed in E. coli - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Source
- E.coli
- Buffer Solution
- 10mM Acetic Acid
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Purity
- >98 % by SDS PAGE and HPLC
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 17.5kDa (153 amino acids)
- Endotoxin Level
- < 0.1 ng/ug
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.2 | 0.4 ng/well |
| Functional Assays | |
0.1ng/ml | 10ng/ml |
| Western Blotting | |
1.5 | 3.0 ng/lane |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
- UniProt
- P13232
- Entrez Gene
- IL7
- GO Terms
- GO:0005125 cytokine activity
- GO:0005139 interleukin-7 receptor binding
- GO:0005615 extracellular space
- GO:0006959 humoral immune response
- GO:0007267 cell-cell signaling
- GO:0009887 organ morphogenesis
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0045453 bone resorption
- View More GO Terms
- GO:0045582 positive regulation of T cell differentiation
PHP046
155864
161387
166193
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-7 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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