IL-4

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Recombinant Human Interleukin-4

Product Type: Recombinant Protein
Specificity: IL-4

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PHP234A Datasheet Datasheet SDS SDS	E FN WB	5 µg		Log in	Get Quote
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PHP234 Datasheet Datasheet SDS SDS	E FN WB	20 µg		Log in	Get Quote
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Interleukin-4, also known as B-cell stimulatory factor 1, is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. Excessive secretion of IL-4 by Th2 helper cells, has been linked with allergy and elevated IgE levels.

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Pack Size: 5 µg
Reconstitute with 50 ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA.

Pack Size: 20 µg
Reconstitute with 200 ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified recombinant IL-4 expressed in E. coli
Source: E.coli
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: The ED₅₀ as determined by the dose-dependent stimulation of human TF-1 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁶ units/mg.
Purity: >98% by SDS PAGE and HPLC
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 14.9 kDa (129 amino acid residues)
Endotoxin Level: < 1.0 EU/ug
Regulatory: For research purposes only
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	0.1	10.00ng/ml
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PHP234 may be used as a standard in ELISA application involving AHP780.
Western Blotting: PHP234 may be used as a positive control for western blotting applications involving AHP780.

UniProt: P05112
Entrez Gene: IL4
GO Terms: GO:0005125 cytokine activity; GO:0005136 interleukin-4 receptor binding; GO:0005615 extracellular space; GO:0006935 chemotaxis; GO:0006968 cellular defense response; GO:0008083 growth factor activity; GO:0008203 cholesterol metabolic process; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; View More GO Terms; GO:0030183 B cell differentiation; GO:0030890 positive regulation of B cell proliferation; GO:0042092 type 2 immune response; GO:0043066 negative regulation of apoptosis; GO:0045189 connective tissue growth factor biosynthetic process; GO:0045348 positive regulation of MHC class II biosynthetic process; GO:0045671 negative regulation of osteoclast differentiation; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048295 positive regulation of isotype switching to IgE isotypes; GO:0048304 positive regulation of isotype switching to IgG isotypes