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IL-1RA

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Recombinant Human Interleukin-1RA

Product Type
Recombinant Protein
Specificity
IL-1RA

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SDS Safety Datasheet SDS
E FN WB 20 µg loader
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Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.

In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40 ng/ml.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.2 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation
Recombinant protein produced in E. coli
Source
E.coli
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution.
Protein Molecular Weight
17 kDa (153 amino acids)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 20 40ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human Interleukin-1RA may be used as the standard for ELISA applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.
Western Blotting
Recombinant huma Interleukin-1RA may be used as a positive control for Western Blotiing applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.

Synonyms
Interleukin-1 Receptor Antagonist
UniProt
P18510
Entrez Gene
IL1RN
GO Terms
GO:0005886 plasma membrane
GO:0005125 cytokine activity
GO:0005152 interleukin-1 receptor antagonist activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005813 centrosome
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0051384 response to glucocorticoid stimulus

PHP080

155294 157574 160138 160913

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