IL-1RA

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Recombinant Human Interleukin-1RA

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PHP080	E FN WB			20 µg

Summary

Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.

In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40ng/ml.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilised
Reconstitution: Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation: Recombinant protein produced in E. coli
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: None present
Purity: >98% by SDS PAGE
Approx. Protein Concentrations: Total protein concentration 0.1mg/ml after reconstitution.
Protein Molecular Weight: 17kD/153 amino acids
Endotoxin Level: <0.1ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution

More Information

UniProt: P18510 Related reagents
Entrez Gene: IL1RN Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005125 cytokine activity; GO:0005152 interleukin-1 receptor antagonist activity; GO:0005615 extracellular space; GO:0005634 nucleus; GO:0005813 centrosome; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0051384 response to glucocorticoid stimulus
Regulatory: For research purposes only

Applications of IL-1RA

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	20	40ng/ml
Western Blotting	1.5	3.0ng/lane

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.

ELISA: Recombinant human Interleukin-1RA may be used as the standard for ELISA applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.
Western Blotting: Recombinant huma Interleukin-1RA may be used as a positive control for Western Blotiing applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.

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