IL-11

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Recombinant Human Interleukin-11

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP303
Datasheet
E
SDS
10 µg loader

Recombinant Human interleukin-11

Interleukin-11 (IL-11), also known as AGIF belongs to the IL-6 family of cytokines, and plays a protective role in acute inflammatory settings (Traber et al. 2019). IL-11 has both pro- and anti-inflammatory functions through activation of signaling cascades (Lokau et al. 2017). IL-11 is largely absent in the body fluids of healthy people, but is upregulated in a series of diseases. IL-11 can be expressed in fibroblasts, and its inhibition has been suggested as a treatment for fibrotic disease (Schafer et al. 2017), including fibrotic cardiovascular diseases (Ye et al. 2019). A role of IL-11 has also been identified in development of inflammation-associated cancers, and its inhibition may be a therapeutic goal (Putoczki et al. 2013).

This recombinant Human interleukin-11 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-11 antibody, clone D08-4C12 (MCA6204GA) as a capture antibody and biotinylated Mouse anti Human interleukin-11 antibody, clone C16-4B8 (MCA6205B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Recombinant protein expressed in E. coli and purified by ion exchange chromatography
Buffer Solution
50 mM Tris-HCl, pH8.0, 0.15 M NaCl
Preservative Stabilisers
1.0% Trehalose
0.05% Tween80
Purity
≥90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
Approx. Protein Concentrations
100 μg/ml after reconstitution
Protein Molecular Weight
19.1 kDa

Storage Information

Storage
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
P20809
Entrez Gene
IL11
GO Terms
GO:0005125 cytokine activity
GO:0005142 interleukin-11 receptor binding
GO:0005615 extracellular space
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0030168 platelet activation
GO:0030183 B cell differentiation
GO:0030219 megakaryocyte differentiation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0043410 positive regulation of MAPKKK cascade
GO:0045444 fat cell differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046888 negative regulation of hormone secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
Regulatory
For research purposes only

Applications of IL-11

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Interleukin-11 MCA6204GA E 0.1 mg loader
Mouse anti Human Interleukin-11:Biotin MCA6205B E 0.1 mg loader

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