IL-10
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Recombinant Human interleukin-10 is a 161 amino acid recombinant IL-10 cytokine, with a range of reported properties including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cells response.
Recombinant human IL-10 has an ED50 of <2.0ng/ml as determined by co-stimulation of MC/9 cells with human IL-4.
Recombinant human IL-10 has an ED50 of <2.0ng/ml as determined by co-stimulation of MC/9 cells with human IL-4.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant IL-10 - lyophilised
- Reconstitution
- Reconstitute with 0.1ml of 5mM sodium phosphate pH 7.2. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
After the initial reconstitution step, the product should be diluted to a working concentration using an aqueous buffer containing 0.1% BSA (or any albumin), or diluted in culture media containing serum such as FCS. - Preparation
- Recombinant protein expressed in Escherichia coli
- Buffer Solution
- Sodium Phosphate
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- 5 x 105 units/mg
- Purity
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- Total protein concentration 0.1mg/ml after reconstitution.
- Protein Molecular Weight
- 18.6 kD
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P22301
- Entrez Gene
- IL10
- GO Terms
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0002904 positive regulation of B cell apoptosis
- GO:0005125 cytokine activity
- GO:0005141 interleukin-10 receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0007253 cytoplasmic sequestering of NF-kappaB
- GO:0007267 cell-cell signaling
- GO:0008083 growth factor activity
- GO:0030183 B cell differentiation
- GO:0030595 leukocyte chemotaxis
- GO:0030889 negative regulation of B cell proliferation
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032800 receptor biosynthetic process
- GO:0042092 type 2 immune response
- GO:0042100 B cell proliferation
- GO:0042130 negative regulation of T cell proliferation
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045191 regulation of isotype switching
- GO:0045347 negative regulation of MHC class II biosynthetic process
- GO:0045355 negative regulation of interferon-alpha biosynthetic process
- GO:0050715 positive regulation of cytokine secretion
- GO:0051045 negative regulation of membrane protein ectodomain proteolysis
- GO:0051091 positive regulation of transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- Regulatory
- For research purposes only
Applications of IL-10
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4ng/well | |
| Functional Assays | 0.2 | 20ng/ml | |
| Western Blotting | 1.5 | 3.0ng/lane |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
- Western Blotting
- PHP047A may be used under reducing or non-reducing western blotting conditions with AHP795.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Human Interleukin-10 | AHP795 | E FN WB | 0.1 mg |
|
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