IL-10

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Recombinant Human Interleukin-10

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP047A Datasheet	E FN WB MSDS	10 µg	Log in		Get Quote

Summary
Useful Reagents

Recombinant Human interleukin-10 is a 161 amino acid recombinant IL-10 cytokine, with a range of reported properties including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cells response.

Recombinant human IL-10 has an ED₅₀ of <2.0ng/ml as determined by co-stimulation of MC/9 cells with human IL-4.

Product Details

Target Species: Human
Product Form: Purified recombinant IL-10 - lyophilised
Reconstitution: Reconstitute with 0.1ml of 5mM sodium phosphate pH 7.2. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

After the initial reconstitution step, the product should be diluted to a working concentration using an aqueous buffer containing 0.1% BSA (or any albumin), or diluted in culture media containing serum such as FCS.
Preparation: Recombinant protein expressed in Escherichia coli
Buffer Solution: Sodium Phosphate
Preservative Stabilisers: None present
Activity: 5 x 10⁵ units/mg
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: Total protein concentration 0.1mg/ml after reconstitution.
Protein Molecular Weight: 18.6 kD
Endotoxin Level: <0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at -20^oC. Following reconstitution store at -20^oC.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P22301 Related reagents
Entrez Gene: IL10 Related reagents
GO Terms: GO:0002740 negative regulation of cytokine secretion involved in immune response; GO:0002904 positive regulation of B cell apoptosis; GO:0005125 cytokine activity; GO:0005141 interleukin-10 receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006954 inflammatory response; GO:0007253 cytoplasmic sequestering of NF-kappaB; GO:0007267 cell-cell signaling; GO:0008083 growth factor activity; GO:0030183 B cell differentiation; GO:0030595 leukocyte chemotaxis; GO:0030889 negative regulation of B cell proliferation; GO:0032715 negative regulation of interleukin-6 production; GO:0032800 receptor biosynthetic process; GO:0042092 type 2 immune response; GO:0042100 B cell proliferation; GO:0042130 negative regulation of T cell proliferation; GO:0043193 positive regulation of gene-specific transcription; GO:0045191 regulation of isotype switching; GO:0045347 negative regulation of MHC class II biosynthetic process; GO:0045355 negative regulation of interferon-alpha biosynthetic process; GO:0050715 positive regulation of cytokine secretion; GO:0051045 negative regulation of membrane protein ectodomain proteolysis; GO:0051091 positive regulation of transcription factor activity; GO:0051384 response to glucocorticoid stimulus
Regulatory: For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	0.2	20ng/ml
Western Blotting	1.5	3.0ng/lane

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.

Western Blotting: PHP047A may be used under reducing or non-reducing western blotting conditions with AHP795.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Human Interleukin-10	AHP795	E FN WB	0.1 mg	Log in		Get Quote

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