IL-10

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Recombinant Human Interleukin-10

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP047A E FN WB 10 µg
Recombinant Human interleukin-10 is a 161 amino acid recombinant IL-10 cytokine, with a range of reported properties including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cells response.

Recombinant human IL-10 has an ED50 of <2.0ng/ml as determined by co-stimulation of MC/9 cells with human IL-4.

Product Details

Target Species
Human
Product Form
Purified recombinant IL-10 - lyophilised
Reconstitution
Reconstitute with 0.1ml of 5mM sodium phosphate pH 7.2. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

After the initial reconstitution step, the product should be diluted to a working concentration using an aqueous buffer containing 0.1% BSA (or any albumin), or diluted in culture media containing serum such as FCS.
Preparation
Recombinant protein expressed in Escherichia coli
Buffer Solution
Sodium Phosphate
Preservative Stabilisers
None present
Activity
5 x 105 units/mg
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution.
Protein Molecular Weight
18.6 kD
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P22301 Related reagents
Entrez Gene
IL10 Related reagents
GO Terms
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005141 interleukin-10 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0030183 B cell differentiation
GO:0030595 leukocyte chemotaxis
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042092 type 2 immune response
GO:0042100 B cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0043193 positive regulation of gene-specific transcription
GO:0045191 regulation of isotype switching
GO:0045347 negative regulation of MHC class II biosynthetic process
GO:0045355 negative regulation of interferon-alpha biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.2 20ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
Western Blotting
PHP047A may be used under reducing or non-reducing western blotting conditions with AHP795.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human Interleukin-10 AHP795 0.1 mg E FN WB