Human IgG4 | AbD00264_hIgG4_Pro

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Recombinant Human IgG4 Lambda (Mutant)

Human IgG4 lambda (mutant) isotype control is a fully human recombinant antibody with the mutation S228P in the core hinge region. It is recommended as a control for monoclonal antibody drugs of the same isotype.

Product Type
Recombinant Protein
IgG4 Lambda
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Recombinant Human IgG4 lambda (mutant), clone AbD00264_hIgG4_Pro is a recombinant human IgG4 antibody with a lambda light chain and a mutation S228P in the core hinge region.

Clone AbD00264_hIgG4_Pro is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG4 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

In addition to the tetrameric IgG structure, composed of two copies each of the heavy and light chains, wild type human IgG4 forms so-called half-molecules. This species contains only one heavy and one light chain. Half-molecules can assemble with an unrelated half-molecule to form bispecific tetrameric antibody species (Aalberse et al. 2002). Half-molecules of human IgG4 preparations can be detected as an additional species under denaturing, non-reducing conditions using SDS-PAGE, size exclusion chromatography or capillary electrophoresis. The serine to proline mutation at amino acid 228 in the core hinge region of IgG4 prevents the formation of half-molecules (Bloom et al. 1997). Recombinant Human IgG4 lambda (mutant) does not display this additional species; half-molecules cannot be detected in SDS-PAGE or by size exclusion chromatography.

In addition to the mutant recombinant, Bio-Rad also offers recombinant Human IgG4 lambda in its wild type form (HCA050). Recombinant Human IgG4 kappa is also available in both wild type (HCA195) and mutant form (HCA247).

Product Details

Product Form
Human IgG4 antibody selected from the HuCAL GOLD phage display library and expressed in a human cell line - liquid
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only

Applications of Human IgG4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Recombinant Human IgG4 Lambda HCA050 E 0.1 mg loader
Recombinant Human IgG4 Kappa HCA195 E 0.1 mg loader
Recombinant Human IgG4 Kappa (Mutant) HCA247 E 0.1 mg loader

Product Specific References

Further Reading

  1. Labrijn, A.F. et al. (2009) Therapeutic IgG4 antibodies engage in Fab-arm exchange with endogenous human IgG4 in vivo.
    Nat Biotechnol. 27 (8): 767-71.
  2. Wilkinson, I.C. et al. (2013) Monovalent IgG4 molecules: immunoglobulin Fc mutations that result in a monomeric structure.
    MAbs. 5 (3): 406-17.
  3. Aalberse RC & Schuurman J (2002) IgG4 breaking the rules.
    Immunology. 105 (1): 9-19.
  4. Bloom, J.W. et al. (1997) Intrachain disulfide bond in the core hinge region of human IgG4
    Protein Sci. 6:407-415

Fluorescent Spectraviewer

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