Recombinant Human IgG4 Lambda (Mutant), clone AbD00264_hIgG4_Pro

Recombinant Human IgG4 lambda (mutant), clone AbD00264_hIgG4_Pro is a recombinant human IgG4 antibody with a lambda light chain and a mutation S228P in the core hinge region.

Clone AbD00264_hIgG4_Pro is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG4 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

In addition to the tetrameric IgG structure, composed of two copies each of the heavy and light chains, wild type human IgG4 forms so-called half-molecules. This species contains only one heavy and one light chain. Half-molecules can assemble with an unrelated half-molecule to form bispecific tetrameric antibody species (Aalberse et al. 2002). Half-molecules of human IgG4 preparations can be detected as an additional species under denaturing, non-reducing conditions using SDS-PAGE, size exclusion chromatography or capillary electrophoresis. The serine to proline mutation at amino acid 228 in the core hinge region of IgG4 prevents the formation of half-molecules (Bloom et al. 1997). Recombinant Human IgG4 lambda (mutant) does not display this additional species; half-molecules cannot be detected in SDS-PAGE or by size exclusion chromatography.

In addition to the mutant recombinant, Bio-Rad also offers recombinant Human IgG4 lambda in its wild type form (HCA050). Recombinant Human IgG4 kappa is also available in both wild type (HCA195) and mutant form (HCA247).

Product Form: Human IgG4 antibody selected from the HuCAL GOLD phage display library and expressed in a human cell line - liquid.
Preparation: Purified IgG prepared by affinity chromatography on Protein G.
Source: HKB-11
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Green fluorescent protein
Approx. Protein Concentrations: Antibody concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Licensed Use: For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

Description	Product Code	Applications	Pack Size	Your Price
Recombinant Human IgG4 Lambda	HCA050	E	0.1 mg	Log in
List Price	Your Price
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Description	Recombinant Human IgG4 Lambda
Recombinant Human IgG4 Kappa	HCA195	E	0.1 mg	Log in
List Price	Your Price
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Description	Recombinant Human IgG4 Kappa
Recombinant Human IgG4 Kappa (Mutant)	HCA247	E	0.1 mg	Log in
List Price	Your Price
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Description	Recombinant Human IgG4 Kappa (Mutant)

HCA246

151624

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