Human IgG3 | AbD00264_hIgG3
Recombinant Human IgG3 lambda (HCA178) used as an antigen in an indirect ELISA followed by Mouse anti Human IgG (5172-6004) and HRP conjugated Rabbit F(ab')2 anti Mouse IgG (STAR13B)
Recombinant Human IgG3 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG3 lambda. The larger peak corresponds to monomeric, fully assembled AbD00264_hIgG3. The smaller shoulder most likely contains dimers and aggregates
Recombinant Human IgG3 Lambda
- Product Type
- Recombinant Protein
- Clone
- AbD00264_hIgG3
- Isotype
- IgG3 Lambda
- Specificity
- Human IgG3
- Region
- LAMBDA
Antibody Quality and Performance Guarantee
| Recombinant Human IgG3 lambda, clone AbD00264_hIgG3 is a recombinant Human IgG3 antibody containing a lambda light chain. The heavy chain belongs to the G3m(b1), previously designated G3m5, allotype. Clone AbD00264_hIgG3 is specific for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent. |
|
- Product Form
- Human IgG3 antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C * E | 0.2 mg |
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| Description | Mouse anti Human IgG (Fc) CH2 Domain:HRP | ||||||
- UniProt
- P01860
- Entrez Gene
- IGHG3
- GO Terms
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
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