Human IgG2 | AbD18705_hIgG2
Recombinant Human IgG2 kappa (HCA193) detected with Mouse anti Human IgG2 followed by
Rabbit F(ab')2 anti Mouse IgG conjugated to HRP (STAR13B).
Recombinant Human IgG2 kappa was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG2 kappa. The larger peak corresponds to monomeric, fully assembled AbD18705_hIgG2. The smaller shoulder most likely contains dimers and aggregates
Recombinant Human IgG2 kappa is a recombinant human IgG2 antibody with a kappa light chain and a heavy chain belonging to the G2m(..), formally known as the G2m(n-) allotype.
Recombinant Human IgG2 kappa is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure human IgG2 kappa levels, or as a control antibody when using other human antibodies of the same isotype and subclass.
Recombinant Human IgG2 kappa forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.
Recombinant Human IgG2 kappa is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure human IgG2 kappa levels, or as a control antibody when using other human antibodies of the same isotype and subclass.
Recombinant Human IgG2 kappa forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.
Product Details
- Product Form
- Human IgG2 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Green Fluorescent Protein (GFP).
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01834
- P01859
- Entrez Gene
- IGKC
- IGHG2
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Human IgG2
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Human IgG2 Lambda | HCA108 | E | 0.1 mg |
|
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
