Human IgG1 | AbD18705it
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG1 N297Q Kappa. The larger peak corresponds to monomeric, fully assembled AbD18705it. The smaller shoulder most likely contains dimers and aggregates.
Recombinant Human IgG1 N297Q Kappa was analyzed under reducing, denaturing conditions (R) and non-reducing, denaturing conditions (NR). Bands were visualized using Coomassie staining and the full IgG (H2L2), heavy (H) and light chains (L) are indicated. The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand side of the image.
Recombinant Human IgG1 N297Q Kappa Allotype G1m3
- Product Type
- Recombinant Protein
- Clone
- AbD18705it
- Isotype
- IgG1 Kappa
- Specificity
- Human IgG1
- Region
- KAPPA ALLOTYPE G1m3
Antibody Quality and Performance Guarantee
| Recombinant Human IgG1 N297Q kappa allotype G1m3, clone AbD18705it is a recombinant human antibody with a kappa light chain, and a heavy chain belonging to the G1m3 allotype, also designated G1m(f). It contains a N297Q mutation in its Fc region (mutation of the asparagine residue at position 297 into a glutamine) which can prevent binding to FcR1. This product is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a relevant control for scientists working on the R&D of therapeutic antibodies with this engineered Fc region. It is recommended that this reagent is used at the same concentration as the test reagent. The main allelic forms of IgG1 are G1m(f), G1m(f,a), and G1m(z,a). The G1m(f) (or G1m3) allele is only found in Caucasoid populations, whereas the G1m(f,a) allele is common in Mongoloids, but other variants, G1m(z,a,x) and G1m(z,a,v), have also been described; for further details on IgG1 allotypes see IgG subclasses and allotypes: from structure to effector functions, and IMGT Repertoire (IG and TR) Allotypes: Human (Homo sapiens) IGHC. The following publication reviews the serologically defined human IgG allotypes and considers the potential for allotype differences to contribute to or potentiate immunogenicity; Human immunoglobulin allotypes, possible implications for immunogenicity Our range of IgG1 controls also includes Recombinant Human IgG1 kappa allotype G1m17,1, and the corresponding allotypes for IgG1 lambda. Another IgG1 control containing a LALA double mutation at positions 234 and 235 that reduces its binding to FcγRI, II and III is also available under product code HCA395. View a summary of all recombinant fully human immunoglobulin isotype controls |
|
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human IgG1-N297Q-FcSpyCatcher3 | TZC026 | IY | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human IgG1-N297Q-FcSpyCatcher3 | ||||||
- UniProt
- P01834
- P01857
- Entrez Gene
- IGKC
- IGHG1
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
HCA394
165222
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