Human IgG1 | AbD00264_hIgG1
Recombinant Human IgG1 Lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG1 Lambda. The larger peak corresponds to monomeric, fully assembled AbD00264_hIgG1. The smaller shoulder most likely contains dimers and aggregates
Recombinant Human IgG1 lambda allotype G1m3, clone AbD00264_hIgG1 is a recombinant human antibody with a lambda light chain, and heavy chain belonging to the G1m3 allotype, also designated G1m(f).
This product is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human IgG1 antibodies of the same isotype or allotype. It is recommended that this reagent is used at the same concentration as the test reagent.
The main allelic forms of IgG1 are G1m(f), G1m(f,a), and G1m(z,a). The G1m(f) (or G1m3) allele is only found in Caucasoid populations, whereas the G1m(f,a) allele is common in Mongoloids, but other variants, G1m(z,a,x) and G1m(z,a,v), have also been described; for further details on IgG1 allotypes see IgG subclasses and allotypes: from structure to effector functions, and IMGT Repertoire (IG and TR) Allotypes: Human (Homo sapiens) IGHC.
The following publication reviews the serologically defined human IgG allotypes and considers the potential for allotype differences to contribute to or potentiate immunogenicity; Human immunoglobulin allotypes, possible implications for immunogenicity
Our range of IgG1 controls also includes Recombinant Human IgG1 lambda allotype G1m17,1, and the corresponding allotypes for IgG1 kappa.
View a summary of all recombinant fully human immunoglobulin isotype controls
This product is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human IgG1 antibodies of the same isotype or allotype. It is recommended that this reagent is used at the same concentration as the test reagent.
The main allelic forms of IgG1 are G1m(f), G1m(f,a), and G1m(z,a). The G1m(f) (or G1m3) allele is only found in Caucasoid populations, whereas the G1m(f,a) allele is common in Mongoloids, but other variants, G1m(z,a,x) and G1m(z,a,v), have also been described; for further details on IgG1 allotypes see IgG subclasses and allotypes: from structure to effector functions, and IMGT Repertoire (IG and TR) Allotypes: Human (Homo sapiens) IGHC.
The following publication reviews the serologically defined human IgG allotypes and considers the potential for allotype differences to contribute to or potentiate immunogenicity; Human immunoglobulin allotypes, possible implications for immunogenicity
Our range of IgG1 controls also includes Recombinant Human IgG1 lambda allotype G1m17,1, and the corresponding allotypes for IgG1 kappa.
View a summary of all recombinant fully human immunoglobulin isotype controls
Product Details
- Product Form
- Human IgG1 antibody selected from the HuCAL GOLD phage display library and expressed in a human cell line, HKB-11 - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Pack Size: 0.1 mg, 50 µgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01857
- Entrez Gene
- IGHG1
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Human IgG1
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
|
|
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
|
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