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Human IgG1 | AbD00264_hIgG1

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Recombinant Human IgG1 Lambda Allotype G1m3, clone AbD00264_hIgG1 thumbnail image 1 Recombinant Human IgG1 Lambda Allotype G1m3, clone AbD00264_hIgG1 thumbnail image 2
Recombinant Human IgG1 Lambda Allotype G1m3, clone AbD00264_hIgG1 gallery image 1
SDS-PAGE analysis of recombinant Human IgG1 Lambda

Recombinant Human IgG1 Lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
Recombinant Human IgG1 Lambda Allotype G1m3, clone AbD00264_hIgG1 gallery image 2
Size exclusion chromatography of recombinant Human IgG1 Lambda

UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG1 Lambda. The larger peak corresponds to monomeric, fully assembled AbD00264_hIgG1. The smaller shoulder most likely contains dimers and aggregates

Recombinant Human IgG1 Lambda Allotype G1m3

Product Type
Recombinant Protein
Clone
AbD00264_hIgG1
Isotype
IgG1 Lambda
Product Code Applications Pack Size List Price Quantity
HCA049A
Datasheet
E
SDS
50 µg loader

HCA049
Datasheet
E
SDS
0.1 mg loader

HCA049G
Datasheet
E
SDS
1 mg loader

Recombinant Human IgG1 lambda, clone AbD00264_hIgG1 contains a lambda light chain. The heavy chain belongs to the G1m(f), previously designated G1m3, allotype.

This product is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.

Product Details

Product Form
Human IgG1 antibody selected from the HuCAL GOLD phage display library and expressed in a human cell line, HKB-11 - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Pack Size: 50 µg, 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
C6KXN3
P01857
Entrez Gene
IGHG1
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgG1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 E WB 0.5 ml loader
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader

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