Human IgE | AbD18705_hIgE

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Recombinant Human IgE Kappa

Product Type
Recombinant Protein
Clone
AbD18705_hIgE
Isotype
IgE Kappa
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA190 E 0.1 mg
Recombinant Human IgE kappa, clone AbD18705_hIgE is a recombinant human IgE antibody with a kappa light chain. Clone AbD18705_hIgE is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgE levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

Recombinant Human IgE kappa, clone AbD18705_hIgE forms part of a range of human recombinant immunoglobulins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.

Product Details

Product Form
Human IgE antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgE prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgE

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human IgE Lambda HCA171 0.1 mg E