Human IgA1 | AbD00264_hIgA1
Recombinant Human IgA1 lambda detected with Mouse anti Human IgA1 alpha chain conjugated to biotin (913008) followed by HRP conjugated Streptavidin (STAR5B)
Recombinant Human IgA1 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgA1 lambda. The larger peak corresponds to monomeric, fully assembled AbD264_hIgA. The smaller shoulder most likely contains dimers and aggregates
Recombinant Human IgA1 Lambda
- Product Type
- Recombinant Protein
- Clone
- AbD00264_hIgA1
- Isotype
- IgA1 Lambda
- Specificity
- Human IgA1
- Region
- LAMBDA
Antibody Quality and Performance Guarantee
| Recombinant Human IgA1 lambda, clone AbD00264_hIgA1 is a recombinant human IgA1 antibody with a lambda light chain. Recombinant Human IgA1 lambda, clone AbD00264_hIgA1 is specific for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent. |
- Product Form
- Human IgA antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgA prepared by affinity chromatography.
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- UniProt
- P01876
- Entrez Gene
- IGHA1
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0006955 immune response
- GO:0018298 protein-chromophore linkage
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA172
0612
156451
165224
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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