Galectin-3

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Recombinant Human Galectin-3

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP188 E FN WB 50 µg
Recombinant Human galectin-3 (Gal-3) is a member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties.

Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumour metastasis, and several studies have shown a correlation between levels of Gal-3 and tumour progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled waterr. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant galectin-3 expressed in E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Activity
Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.
Purity
>98% by SDS PAGE/HPLC
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
26 kDa (250 amino acid residues)
Endotoxin Level
< 1 EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P17931 Related reagents
Entrez Gene
LGALS3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005529 sugar binding
GO:0005634 nucleus
GO:0005743 mitochondrial inner membrane
GO:0019863 IgE binding
GO:0030154 cell differentiation
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of Galectin-3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP188 may be used as a standard in ELISA applications involving AHP1481B.
Western Blotting
PHP188 may be used as a positive control in western blotting applications involving AHP1481B.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human Galectin-3:Biotin AHP1481B 50 µg E WB