Galectin-3

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Recombinant Human Galectin-3

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP188 Datasheet	E FN WB SDS	50 µg	Log in		Get Quote

Recombinant Human galectin-3 (Gal-3) is a member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties.

Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumour metastasis, and several studies have shown a correlation between levels of Gal-3 and tumour progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 0.5 ml distilled waterr. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified recombinant galectin-3 expressed in E.coli
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.
Purity: >98% by SDS PAGE/HPLC
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 26 kDa (250 amino acid residues)
Endotoxin Level: < 1.0 EU/ug

Storage Information

Storage: Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P17931
Entrez Gene: LGALS3
GO Terms: GO:0005886 plasma membrane; GO:0005529 sugar binding; GO:0005634 nucleus; GO:0005743 mitochondrial inner membrane; GO:0019863 IgE binding; GO:0030154 cell differentiation; GO:0045087 innate immune response
Regulatory: For research purposes only

Applications of Galectin-3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: PHP188 may be used as a standard in ELISA applications involving AHP1481B.
Western Blotting: PHP188 may be used as a positive control in western blotting applications involving AHP1481B.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Human Galectin-3:Biotin	AHP1481B	E WB	50 µg	Log in		Get Quote

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