Galectin-3
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Recombinant Human Galectin-3
- Product Type
- Recombinant Protein
- Specificity
- Galectin-3
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| Recombinant Human galectin-3 (Gal-3) is a member of the galectin family of beta-galactoside-binding proteins, involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties. Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumor metastasis, and expression correlates with tumor progression in liver, thyroid, colon, gastric and breast carcinomas. |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled waterr. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preparation
- Purified recombinant galectin-3 expressed in E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.
- Purity
- >98% by SDS PAGE/HPLC
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 26 kDa (250 amino acid residues)
- Endotoxin Level
- < 1.0 EU/ug
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.2 | 0.4ng/well |
| Functional Assays | |
||
| Western Blotting | |
1.5 | 3.0ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Human Galectin-3:Biotin | AHP1481B | E WB | 50 µg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Human Galectin-3:Biotin | ||||||
- Synonyms
- MAC-2
- UniProt
- P17931
- Entrez Gene
- LGALS3
- GO Terms
- GO:0005886 plasma membrane
- GO:0005529 sugar binding
- GO:0005634 nucleus
- GO:0005743 mitochondrial inner membrane
- GO:0019863 IgE binding
- GO:0030154 cell differentiation
- GO:0045087 innate immune response
View more products with GALECTIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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