Galectin-3

Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumour metastasis, and several studies have shown a correlation between levels of Gal-3 and tumour progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled waterr. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preparation
- Purified recombinant galectin-3 expressed in E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.
- Purity
- >98% by SDS PAGE/HPLC
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 26 kDa (250 amino acid residues)
- Endotoxin Level
- < 1.0 EU/ug
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P17931
- Entrez Gene
- LGALS3
- GO Terms
- GO:0005886 plasma membrane
- GO:0005529 sugar binding
- GO:0005634 nucleus
- GO:0005743 mitochondrial inner membrane
- GO:0019863 IgE binding
- GO:0030154 cell differentiation
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of Galectin-3
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.2 | 0.4ng/well | |
Functional Assays | |||
Western Blotting | 1.5 | 3.0ng/lane |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Human Galectin-3:Biotin | AHP1481B | E WB | 50 µg |
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