Galectin-1

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Recombinant Human Galectin-1

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP187 E FN WB 50 µg
Recombinant Human galectin-1 (Gal-1) is a member of the galectin family of beta-galactoside-binding proteins, which exhibits both intracellular and extracellular functions, and is differentially expressed by a wide range of normal and neoplastic tissues.

Galectin-1 has been implicated in several major cellular functions including proliferation, motility, haematopoietic cell differentiation, apoptosis promotion and immunoregulatory effects. The expression of Gal-1 by neuronal and glial cells correlates with neuroregeneration following injury, and studies have shown the importance of Gal-1 to primary sensory neurons and synaptic connections in the spinal cord, during embryonic development.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the additiona of 0.1% bovine serum albumin (BSA) is recommended.
Preparation
Purified recombinant galectin-1 expressed in E.coli
Buffer Solution
10mM Sodium phosphate pH7.5
Preservative Stabilisers
None present
Activity
Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.
Purity
>98% by SDS PAGE/HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Molecular Weight
14.5kD (134 amino acid residues)
Endotoxin Level
< 1 EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P09382 Related reagents
Entrez Gene
LGALS1 Related reagents
GO Terms
GO:0016936 galactoside binding
GO:0006915 apoptosis
GO:0004871 signal transducer activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0042981 regulation of apoptosis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
Regulatory
For research purposes only

Applications of Galectin-1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human galectin-1 may be used as the standard for ELISA applications with either our purified human galectin-1 antibody (AHP1480) or our biotinylated human galectin-1 antibody (AHP1480B)
Western Blotting
Recombinant human galectin-1 may be used as the positive control for Western Blotting applications with either our purified human galectin-1 antibody (AHP1480) or our biotinylated human galectin-1 antibody (AHP1480B).