Collagen IV

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Native Human Collagen IV

Product Type: Purified Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
2150-0150	E			0.1 mg

Summary
References (5)

Human collagen type IV from placenta.

Human collagen type I-III, V, VI (<5%). Non collagen proteins (<0.5%).

Product Details

Target Species: Human
Product Form: Purified protein - liquid
Preparation: Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Purity: SDS PAGE: >95%
Approx. Protein Concentrations: Total protein concentration 1.0mg/ml.
Molecular Weight: 340 kDa

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P02462 Related reagents; P08572 Related reagents; Q01955 Related reagents; P53420 Related reagents; P29400 Related reagents; Q14031 Related reagents
Entrez Gene: COL4A1 Related reagents; COL4A2 Related reagents; COL4A5 Related reagents; COL4A4 Related reagents; COL4A3 Related reagents; COL4A6 Related reagents
GO Terms: GO:0001525 angiogenesis; GO:0005201 extracellular matrix structural constituent; GO:0007411 axon guidance; GO:0048407 platelet-derived growth factor binding; GO:0005515 protein binding; GO:0005587 collagen type IV; GO:0016525 negative regulation of angiogenesis; GO:0030198 extracellular matrix organization; GO:0005605 basal lamina; GO:0032836 glomerular basement membrane development
Regulatory: For research purposes only

Applications of Collagen IV

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Collagen IV

Takahashi, S. et al. (2015) C-type lectin-like domain and fibronectin-like type II domain of phospholipase A2 receptor 1 modulate binding and migratory responses to collagen.
FEBS Lett. 589 (7): 829-35.