Collagen IV
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Human collagen type IV from placenta.Human collagen type I-III, V, VI (<5%). Non collagen proteins (<0.5%).
Product Details
- Target Species
- Human
- Product Form
- Purified protein - liquid
- Preparation
- Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Purity
- >95% by SDS-PAGE
- Approx. Protein Concentrations
- Total protein concentration 1.0 mg/ml
- Protein Molecular Weight
- 340 kDa
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02462
- P08572
- Q01955
- P53420
- P29400
- Q14031
- Entrez Gene
- COL4A1
- COL4A2
- COL4A5
- COL4A4
- COL4A3
- COL4A6
- GO Terms
- GO:0001525 angiogenesis
- GO:0005201 extracellular matrix structural constituent
- GO:0007411 axon guidance
- GO:0048407 platelet-derived growth factor binding
- GO:0005515 protein binding
- GO:0005587 collagen type IV
- GO:0016525 negative regulation of angiogenesis
- GO:0030198 extracellular matrix organization
- GO:0005605 basal lamina
- GO:0032836 glomerular basement membrane development
- Regulatory
- For research purposes only
Applications of Collagen IV
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Product Specific References
References for Collagen IV
-
Takahashi, S. et al. (2015) C-type lectin-like domain and fibronectin-like type II domain of phospholipase A2 receptor 1 modulate binding and migratory responses to collagen.
FEBS Lett. 589 (7): 829-35.
Further Reading
-
Glanville, R.W. et al. (1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains.
Eur J Biochem. 95 (2): 383-9. -
Sage, H. & Bornstein, P. (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
Biochemistry. 18 (17): 3815-22. -
Klasson, S.C. et al. (1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
Coll Relat Res. 6 (5): 397-408. -
Bornstein, P. & Sage, H. (1980) Structurally distinct collagen types.
Annu Rev Biochem. 49: 957-1003.
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