CNTF
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Recombinant Human CNTF
- Product Type
- Recombinant Protein
- Specificity
- CNTF
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| Recombinant Human CNTF is a 199 amino acid, Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy. |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 0.2 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
- Preparation
- Purified recombinant CTNF expressed in E. Coli
- Buffer Solution
- 10mM Citric Acid + 75mM NaCl
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
- Purity
- >98% by SDS PAGE/HPLC
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 22.7 kDa
- Endotoxin Level
- < 1.0 EU/ug
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.2 | 0.4ng/well |
| Functional Assays | |
50 | 150ng/ml |
| Western Blotting | |
1.5 | 3.0ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Recombinant human CNTF may be used as a standard for ELISA applications with purified human CNTF antibody (AHP1277).
- Western Blotting
- Recombinant human CNTF may be used as the positive control for Western Blotting applications with purified human CNTF antibody (AHP1277).
- Synonyms
- Ciliary Neurotrophic Factor
- UniProt
- P26441
- Entrez Gene
- CNTF
- GO Terms
- GO:0005127 ciliary neurotrophic factor receptor binding
- GO:0005138 interleukin-6 receptor binding
- GO:0005615 extracellular space
- GO:0040007 growth
- GO:0008083 growth factor activity
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
View more products with CNTF specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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