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CNTF

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Recombinant Human CNTF

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP173
Datasheet
E FN WB
MSDS
20 µg loader

Recombinant Human CNTF is a 199 amino acid, Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.2 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation
Purified recombinant CTNF expressed in E. Coli
Buffer Solution
10mM Citric Acid + 75mM NaCl
Preservative Stabilisers
None present
Activity
The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
Purity
>98% by SDS PAGE/HPLC
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
22.7 kDa
Endotoxin Level
< 1.0 EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
3 months from date of reconstitution

More Information

UniProt
P26441
Entrez Gene
CNTF
GO Terms
GO:0005127 ciliary neurotrophic factor receptor binding
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
Regulatory
For research purposes only

Applications of CNTF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 50 150ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human CNTF may be used as a standard for ELISA applications with purified human CNTF antibody (AHP1277).
Western Blotting
Recombinant human CNTF may be used as the positive control for Western Blotting applications with purified human CNTF antibody (AHP1277).
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