CNTF

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Recombinant Human CNTF

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PHP173	E FN WB			20 µg

Summary

Recombinant Human CNTF is a 199 amino acid, Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilised
Reconstitution: Reconstitute with 0.2ml of 5mM sodium phosphate, pH 7.5.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation: Purified recombinant CTNF expressed in E. Coli
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present.
Activity: The ED₅₀ as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
Purity: >98% by SDS PAGE/HPLC analysis
Approx. Protein Concentrations: Total protein concentration 0.1mg/ml after reconstitution
Molecular Weight: 22.7kD
Endotoxin Level: <1EU/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

More Information

UniProt: P26441 Related reagents
Entrez Gene: CNTF Related reagents
GO Terms: GO:0005127 ciliary neurotrophic factor receptor binding; GO:0005138 interleukin-6 receptor binding; GO:0005615 extracellular space; GO:0040007 growth; GO:0008083 growth factor activity; GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein; GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
Regulatory: For research purposes only

Applications of CNTF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	50	150ng/ml
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: Recombinant human CNTF may be used as a standard for ELISA applications with purified human CNTF antibody (AHP1277).
Western Blotting: Recombinant human CNTF may be used as the positive control for Western Blotting applications with purified human CNTF antibody (AHP1277).

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