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C-Reactive Protein

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ELISA analysis of C-reactive protein using Mouse anti C-reactive protein (MCA5880) as a capture reagent and biotinylated Mouse anti C-reactive protein (MCA5882G) as a detection reagent with native human C-reactive protein (PHP277) as antigen. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 125- (Green), 250- (Red) , and 500- (Blue) fold dilutions.

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Native Human C-Reactive Protein

Product Type
Purified Protein
Specificity
C-Reactive Protein

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Product Code Applications Pack Size List Price Your Price Qty
PHP277
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Puriified Human C-reactive protein is an acute phase protein, produced by the liver that is used as a marker of inflammation and for monitoring and predicting coronary artery disease. C-reactive protein exists in the blood as a large pentamer of approximately 114 kDa.

Target Species
Human
Product Form
Purified protein - liquid
Preparation
0.2 µM sterile filtered
Buffer Solution
Tris buffered saline, 5 mM CaCl2.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Purity
>99%
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
114 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Synonyms
CRP
UniProt
P02741
Entrez Gene
CRP
GO Terms
GO:0005515 protein binding
GO:0006953 acute-phase response
GO:0046872 metal ion binding
GO:0008228 opsonization
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010888 negative regulation of lipid storage
GO:0030169 low-density lipoprotein particle binding
GO:0033265 choline binding
GO:0051637 Gram-positive bacterial cell surface binding

PHP277

150549 159109 159952 162203 163420 180521

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