apo-serum Amyloid A1

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Recombinant Human apo-serum Amyloid A1

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP245 FN 50 µg
Serum Amyloid A (apo-SAA) is an acute phase response protein and injury specific apolipoprotein, synthesized primarily in the liver, which is found in trace amounts in association with high density lipoproteins (HDL) in human serum, but increases dramatically during an acute response to inflammation, infection, trauma, and malignant neoplasia, to become the most abundant HDL apolipoprotein. The acute phase isoforms of apo-SAA are encoded by the genes SAA1 (Apo-SAA1) and SAA2 (Apo-SAA2), and both include allelic variants.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% BSA is recommended.
Preparation
Purified recombinant apo-SAA1 expressed in E.coli
Buffer Solution
5mM Tris, pH7.6
Preservative Stabilisers
None present
Activity
Determined by its ability to chemoattract human monocytes using a range of 10.0-10.0 ng/ml.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
11.7 kDa (105 amino acid residues)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution

More Information

UniProt
P02735 Related reagents
Entrez Gene
SAA1 Related reagents
Regulatory
For research purposes only

Applications of apo-serum Amyloid A1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.