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Adiponectin

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Recombinant Human Adiponectin thumbnail image 1 Recombinant Human Adiponectin thumbnail image 2
Recombinant Human Adiponectin gallery image 1
Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4652) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.
Recombinant Human Adiponectin gallery image 2
Sandwich ELISA analysis of adiponectin binding using Mouse anti Human adiponectin (MCA4653) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.

Recombinant Human Adiponectin

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP196
Datasheet
E FN
SDS
25 µg loader

Recombinant Adiponectin producted in Hi5 cells derived from the cabbage looper moth Trichoplusia ni corresponds to amino acids 19-244 of the human adiponectin precurson and containing an N-terminal histidine tag. Adiponectin is an adipokine exclusively secreted by adipocytes, which plays a major role in the control of fat metabolism and insulin sensitivity, through the regulation of energy homeostasis and glucose and lipid metabolism. A reduced level of plasma adiponectin is associated with type 2 diabetes and obesity insulin resistance. Adiponectin is an abundant protein in normal serum corresponding to approximately 0.1% of the total protein content.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 25 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant adiponectin expressed in High-5 insect cells using a baculovirus expression system
Buffer Solution
10mM TRIS, pH 8.5
75mM L-Arginine
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 3.0-6.0 μg/ml.
Purity
>97% by SDS PAGE and HPLC
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Protein Molecular Weight
26 kDa (226 amino acid residues)
Endotoxin Level
< 1.0 EU/ug

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
Q15848
Entrez Gene
ADIPOQ
GO Terms
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006006 glucose metabolic process
GO:0006091 generation of precursor metabolites and energy
GO:0006635 fatty acid beta-oxidation
GO:0009749 response to glucose stimulus
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0010875 positive regulation of cholesterol efflux
GO:0046326 positive regulation of glucose import
GO:0030853 negative regulation of granulocyte differentiation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033034 positive regulation of myeloid cell apoptosis
GO:0034115 negative regulation of heterotypic cell-cell adhesion
GO:0034383 low-density lipoprotein particle clearance
GO:0034612 response to tumor necrosis factor
GO:0042593 glucose homeostasis
GO:0042803 protein homodimerization activity
GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043499 eukaryotic cell surface binding
GO:0045599 negative regulation of fat cell differentiation
GO:0045650 negative regulation of macrophage differentiation
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0045721 negative regulation of gluconeogenesis
GO:0045923 positive regulation of fatty acid metabolic process
GO:0050728 negative regulation of inflammatory response
GO:0050765 negative regulation of phagocytosis
Regulatory
For research purposes only

Applications of Adiponectin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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