Recombinant Adiponectin producted in Hi5 cells derived from the cabbage looper moth Trichoplusia ni corresponds to amino acids 19-244 of the human adiponectin precurson and containing an N-terminal histidine tag. Adiponectin is an adipokine exclusively secreted by adipocytes, which plays a major role in the control of fat metabolism and insulin sensitivity, through the regulation of energy homeostasis and glucose and lipid metabolism. A reduced level of plasma adiponectin is associated with type 2 diabetes and obesity insulin resistance. Adiponectin is an abundant protein in normal serum corresponding to approximately 0.1% of the total protein content.
- Target Species
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitute with 25 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified recombinant adiponectin expressed in High-5 insect cells
- Buffer Solution
- 10mM TRIS, pH 8.5
- Preservative Stabilisers
- None present
- Carrier Free
- Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 3.0-6.0 μg/ml.
- >97% by SDS PAGE and HPLC
- Approx. Protein Concentrations
- 1.0 mg/ml after reconstitution
- Protein Molecular Weight
- 26 kDa (226 amino acid residues)
- Endotoxin Level
- < 1.0 EU/ug
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
- Entrez Gene
- GO Terms
glucose metabolic process
generation of precursor metabolites and energy
fatty acid beta-oxidation
response to glucose stimulus
positive regulation of protein kinase A signaling cascade
negative regulation of macrophage derived foam cell differentiation
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of cholesterol efflux
positive regulation of glucose import
negative regulation of granulocyte differentiation
negative regulation of tumor necrosis factor production
positive regulation of myeloid cell apoptosis
negative regulation of heterotypic cell-cell adhesion
low-density lipoprotein particle clearance
response to tumor necrosis factor
protein homodimerization activity
negative regulation of I-kappaB kinase/NF-kappaB cascade
eukaryotic cell surface binding
negative regulation of fat cell differentiation
negative regulation of macrophage differentiation
negative regulation of low-density lipoprotein particle receptor biosynthetic process
negative regulation of gluconeogenesis
positive regulation of fatty acid metabolic process
negative regulation of inflammatory response
negative regulation of phagocytosis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Adiponectin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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