TNF Alpha

Recombinant Dog TNF Alpha

Product Type
Recombinant Protein
Specificity
TNF Alpha

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PDP001
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Canine tumor necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the canine inflammatory response. Recombinant dog TNF alpha has an ED50 of 10 pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of actinomycin D.

Target Species
Dog
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Further dilutions should be made in a buffer containing a carrier protein.
Preparation
Recombinant protein expressed in Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
17.3 kDa
Endotoxin Level
< 1.1 EU/ug
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for TNF Alpha

  1. Grob, D. et al. (2021) Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
    Biology. 10 (5): 427.

UniProt
P51742
Entrez Gene
TNF
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
View more products with TNF ALPHA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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