TNF Alpha
Recombinant canine TNF-α (PDP001) used to stimulate canine aortic endothlial cells in vitro prior to protein extraction and western blotting.
Image caption:
Adhesion molecule protein expression in A. vasorum antigen (AvAg)-stimulated canine endothelial cells. CAEC cells were stimulated with A. vasorum L3 antigen (AvAg; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation, total protein was extracted. Non-stimulated CAEC served as negative controls. The expression of E-selectin, P-selectin and VCAM-1 was studied by Western blotting (A); representative illustration of one CAEC isolate and densitometric analysis of protein bands. TNF-α was used as positive control of CAEC stimulation. The detection of vinculin was used as loading control for sample normalization.
From: Grob D, Conejeros I, López-Osorio S, Velásquez ZD, Segeritz L, Gärtner U, Schaper R, Hermosilla C, Taubert A.
Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
Biology. 2021; 10(5):427.
doi: 10.3390/biology10050427
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Dog TNF Alpha
- Product Type
- Recombinant Protein
- Specificity
- TNF Alpha
Antibody Quality and Performance Guarantee
| Canine tumor necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the canine inflammatory response. Recombinant dog TNF alpha has an ED50 of 10 pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of actinomycin D. |
- Target Species
- Dog
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Further dilutions should be made in a buffer containing a carrier protein. - Preparation
- Recombinant protein expressed in Pichia pastoris
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 17.3 kDa
- Endotoxin Level
- < 1.1 EU/ug
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Functional Assays |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for TNF Alpha
-
Grob, D. et al. (2021) Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
Biology. 10 (5): 427.
- UniProt
- P51742
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
View more products with TNF ALPHA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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