IL-1 Beta

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Recombinant Dog Interleukin-1 beta

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PDP014 E WB 5 µg
Recombinant Dog interleukin-1 beta (IL-1β) is a recombinant protein corresponding to a 152 amino acid sequence of native canine IL-1β.

Interleukin-1β is a pleiotropic pro-inflammatory cytokine and member of the IL-1 superfamily. It is secreted by activated macrophages and monocytes and is involved in the modulation of a range of immune and inflammatory responses.

IL-1β and the closely related IL-1α, are both involved in haematopoiesis and exhibit many identical immunomodulatory properties, binding to the same type I interleukin-1 receptor, also known as CD121a.

Product Details

Target Species
Dog
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
17.5 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q98292 Related reagents
Regulatory
For research purposes only

Applications of IL-1 Beta

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2980 as a coating antibody and AHP2980B as a detection antibody.