TNF Alpha

100% Secure

Recombinant Bovine TNF Alpha

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP005 E FN 20 µg
Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response.

Bovine Tumour Necrosis Factor alpha (PBP005) has an ED50 in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.

Product Details

Target Species
Bovine
Product Form
Purified Recombinant Protein- lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water.

Further dilutions should be made in a buffer containing carrier protein.

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
17.4kD
Endotoxin Level
less than 0.04 EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q06599 Related reagents
Entrez Gene
TNF Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of TNF Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
ELISA
PBP005 may be used as a standard with MCA2334 or MCA2335B.

Application Based External Images

ELISA

Product Specific References

References for TNF Alpha

  1. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.