Recombinant Bovine TNF Alpha
- Product Type
- Recombinant Protein
- TNF Alpha
|Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response.
Bovine Tumour Necrosis Factor alpha (PBP005) has an ED50 in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.
- Target Species
- Product Form
- Purified Recombinant Protein- lyophilized
- Reconstitute with 0.5 ml distilled water.
Further dilutions should be made in a buffer containing carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Recombinant protein expressed in Pichia pastoris
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- Endotoxin Level
- < 0.06 EU/ug
- For research purposes only
- 12 months from date of despatch
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
References for TNF Alpha
Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 42: 49.
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