TNF Alpha

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Recombinant Bovine TNF Alpha

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
20 µg loader

Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response.

Bovine Tumour Necrosis Factor alpha (PBP005) has an ED50 in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.

Product Details

Target Species
Product Form
Purified Recombinant Protein- lyophilized
Reconstitute with 0.5 ml distilled water.

Further dilutions should be made in a buffer containing carrier protein.

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Recombinant protein expressed in Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
>95% by SDS PAGE analysis
Protein Molecular Weight
Endotoxin Level
< 0.06 EU/ug

Storage Information

Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
For research purposes only

Applications of TNF Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
PBP005 may be used as a standard with MCA2334 or MCA2335B.

Application Based External Images


Product Specific References

References for TNF Alpha

  1. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.

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