IL-6

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Recombinant Chicken Interleukin-6

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PAP003 FN IA 50 µg
Recombinant chicken inerleukin-6 is a histidine tagged molecule produced in E. coli. Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine, produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells. It plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses.

Recombinant chicken inerleukin-6 induces corticosterone secretion in vivo and induces proliferation of the IL-6 dependant murine hybridoma cell line 7TD1 in vitro (Marais et al. 2011).

Recombinant chicken inerleukin-6 (PAP003) is the immunogen used to produce Bio-Rad chicken IL-6 antibody (AHP942Z).

Product Details

Target Species
Chicken
Product Form
Purified His-tagged recombinant protein expressed in E.coli - liquid
Preparation
Recombinant protein prepared from E. coli.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>85% by Ni Chelate chromatography
Approx. Protein Concentrations
500 ug/ml
Protein Molecular Weight
23.2kD

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of despatch.

More Information

UniProt
Q90YI0 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of IL-6

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
In vitro Assay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IL-6

  1. Marais, M. et al. (2011) Brain IL-6- and PG-dependent actions of IL-1β and lipopolysaccharide in avian fever.
    Am J Physiol Regul Integr Comp Physiol. 301 (3): R791-800.
  2. Ferdous F & Scott T (2015) Bacterial and viral induction of chicken thrombocyte inflammatory responses.
    Dev Comp Immunol. 49 (2): 225-30.
  3. Abdel-mageed, A.M. et al. (2017) Modulatory roles of proinflammatory cytokines on the expression of cathelicidins in the lower regions of the oviduct of laying hens.
    Cytokine. 99: 66-72.
  4. Ferdous, F. & Scott, T. (2015) Bacterial and viral induction of chicken thrombocyte inflammatory responses.
    Dev Comp Immunol. 49 (2): 225-30.

Further Reading

  1. Schneider, K. et al. (2001) Chicken interleukin-6. cDNA structure and biological properties.
    Eur J Biochem. 268 (15): 4200-6.