Recombinant Chicken Interleukin-6

Recombinant chicken inerleukin-6 is a histidine tagged molecule produced in E. coli. Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine, produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells. It plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses.

Recombinant chicken inerleukin-6 induces corticosterone secretion in vivo and induces proliferation of the IL-6 dependant murine hybridoma cell line 7TD1 in vitro (Marais et al. 2011).

Recombinant chicken inerleukin-6 (PAP003) is the immunogen used to produce Bio-Rad chicken IL-6 antibody (AHP942Z).

Target Species: Chicken
Product Form: Purified His-tagged recombinant protein expressed in E.coli - liquid
Preparation: Recombinant protein prepared from E. coli.
Source: E.coli
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Purity: >85% by Ni Chelate chromatography
Approx. Protein Concentrations: 500 ug/ml
Protein Molecular Weight: 23.2 kD
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: His-tag is a registered trademark of EMD Biosciences.

Store at -20^oC only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays
In vitro Assay

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IL-6

Marais, M. et al. (2011) Brain IL-6- and PG-dependent actions of IL-1β and lipopolysaccharide in avian fever.
Am J Physiol Regul Integr Comp Physiol. 301 (3): R791-800.
Ferdous F & Scott T (2015) Bacterial and viral induction of chicken thrombocyte inflammatory responses.
Dev Comp Immunol. 49 (2): 225-30.
Abdel-mageed, A.M. et al. (2017) Modulatory roles of proinflammatory cytokines on the expression of cathelicidins in the lower regions of the oviduct of laying hens.
Cytokine. 99: 66-72.

PAP003

150736

162109

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