IL-10

100% Secure

Recombinant Chicken Interleukin-10

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PAP013
Datasheet
E WB
MSDS
5 µg loader

Recombinant Chicken Interleukin-10 (IL-10) is a recombinant protein corresponding to aa 22-175 of native chicken IL-10.

IL-10 is a member of the IL-10 family consisting of IL-10, IL-19, IL-20, IL-22, IL-24, IL-26, IL-28Α, IL-28Β, and IL-29. IL-10 is a major immune regulatory cytokine produced primarily by monocytes. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα.

Product Details

Target Species
Chicken
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
18.3 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Guarantee
12 months from date of despatch

More Information

UniProt
Q6A2H4
Entrez Gene
IL10
GO Terms
GO:0002826 negative regulation of T-helper 1 type immune response
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
GO:0042832 defense response to protozoan
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045077 negative regulation of interferon-gamma biosynthetic process
Regulatory
For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.