IL-10

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Recombinant Chicken Interleukin-10

Product Type: Recombinant Protein
Specificity: IL-10

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Recombinant Chicken Interleukin-10 (IL-10) is a recombinant protein corresponding to aa 22-175 of native chicken IL-10.

IL-10 is a member of the IL-10 family consisting of IL-10, IL-19, IL-20, IL-22, IL-24, IL-26, IL-28Α, IL-28Β, and IL-29. IL-10 is a major immune regulatory cytokine produced primarily by monocytes. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα.

Target Species: Chicken
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source: Pichia pastoris
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10% Trehalose
Purity: >95% by SDS PAGE analysis
Protein Molecular Weight: 18.3 kDa
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt: Q6A2H4
Entrez Gene: IL10
GO Terms: GO:0002826 negative regulation of T-helper 1 type immune response; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0006955 immune response; GO:0042832 defense response to protozoan; GO:0045019 negative regulation of nitric oxide biosynthetic process; GO:0045077 negative regulation of interferon-gamma biosynthetic process