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TNF Alpha

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Cat TFN alpha ELISA

Recombinant Cat TFN alpha (PFP005) detected using Rabbit anti Cat TFN alpha (AHP2835) as the capture reagent and Rabbit anti Cat TFN alpha:Biotin (AHP2835B) as the detection reagent followed by Streptavidin:HRP.

Recombinant Cat TNF Alpha

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PFP005
Datasheet
E WB
MSDS
5 µg loader

Recombinant Cat TNF Alpha is a recombinant protein corresponding to aa 165-321 of native cat TNF alpha.

TNF alpha is produced by activated macrophages, monocytes, endothelial cells, fibroblast and both T and B lymphocytes. TNF alpha functions in the regulation of immune cells, is able to induce fever, apoptotic cell death, sepsis, cacheria, inflammation and to inhibit tumorigenesis and viral replication.

Product Details

Target Species
Cat
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
17.2 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Guarantee
12 months from date of despatch

More Information

UniProt
A0A0U5EMB8
Regulatory
For research purposes only

Applications of TNF Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2835 as a coating antibody and AHP2835B as a detection antibody.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
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