TNF Alpha

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Recombinant Cat TNF Alpha

Product Type: Recombinant Protein
Specificity: TNF Alpha

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PFP005 Datasheet Datasheet SDS SDS	E WB	5 µg		Log in	Get Quote
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Recombinant Cat TNF Alpha is a recombinant protein corresponding to aa 165-321 of native cat TNF alpha.

TNF alpha is produced by activated macrophages, monocytes, endothelial cells, fibroblast and both T and B lymphocytes. TNF alpha functions in the regulation of immune cells, is able to induce fever, apoptotic cell death, sepsis, cacheria, inflammation and to inhibit tumorigenesis and viral replication.

Target Species: Cat
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source: Pichia pastoris
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10% Trehalose
Purity: >95% by SDS PAGE analysis
Protein Molecular Weight: 17.2 kDa
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2835 as a coating antibody and AHP2835B as a detection antibody.