Streptolysin O is a sulfhydryl-activated toxin produced by groups A, C and G of streptococci. It is an oxygen-labile hemolytic enzyme capable of causing lysis in animal cells, particularly white and red blood cells. It does so by binding to membrane cholesterol, where it polymerises to form a transmembrane channel.
- Target Species
- Product Form
- Recombinant purified Streptolysin O - liquid
- Recombinant Streptolysin O from transformed Escherichia coli. The preparation was purified by metal affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- None present
- Total antigenic activity approx (internal indirect method): 8880 IU/ml
Specific antigenic activity approx: 2596 IU/mg of protein
- Approx. Protein Concentrations
- Current, batch-specific concentration 3.42 mg/ml
- Store at -70oC or -20oC for short term storage.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
- Guaranteed until date of expiry. Please see product label.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Streptolysin-O
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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