Protein G

Recombinant Protein G:HRP

Product Type
Recombinant Protein
Specificity
Protein G

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740605
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Recombinant Protein G is a preparation of streptococcal protein G expressed in E. coli. Protein G binds to the Fc portion of immunoglobulin G subclasses (IgGs) from a broad range of species including human and mouse, and does not bind IgA, IgE, IgM or serum albumin.

Target Species
Bacterial
Product Form
Recombinant protein - conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Recombinant protein G from Streptococcus strain G148, expressed in E.coli.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50% Glycerol
Protein Molecular Weight
22 kDa (monomeric protein lacking cystine residues)
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4000 1/8000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Protein G

  1. Saegerman et al (2004) Evalution of three serum i-ELISAs using monoclonal antibodies and protein G as peroxidase conjugate for the diagnosis of bovine brucellosis.
    Vet Microbiol. 20. 91-105.
  2. Qi et al (2001) Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins.
    Biochem Biophys Methods. 49. 263-273.
  3. McDonald, J.U. et al. (2016) Development of a custom pentaplex sandwich immunoassay using Protein-G coupled beads for the Luminex® xMAP® platform.
    J Immunol Methods. pii: S0022-1759(16)30033-3. [Epub ahead of print]
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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