Escherichia coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
- Target Species
- Product Form
- Purified protein from Escherichia coli - lyophilised
- Reconstitute with 1.0ml sterile distilled water or aqueous buffer of choice.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 74% Sucrose
- >600 U/mg proteinOne unit hydrolyses 1 µmole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.
- Approx. Protein Concentrations
- Protein content is approximately 20-25% of total weight
- Molecular Weight
- 540 kD
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 12 months from date of reconstitution.
- P00722 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0004565 beta-galactosidase activity
- GO:0030246 carbohydrate binding
- GO:0046872 metal ion binding
- GO:0005990 lactose catabolic process
- GO:0009341 beta-galactosidase complex
- For research purposes only
Applications of Beta Galactosidase
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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