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Beta Galactosidase

ISO 9001 Quality Assurance Manufacturing | Print
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Native Escherichia coli Beta Galactosidase

Product Type
Purified Protein
Specificity
Beta Galactosidase

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4600-1704
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Escherichia coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.

Target Species
Bacterial
Product Form
Purified protein from Escherichia coli - lyophilized
Reconstitution
Reconstitute with 1.0 ml sterile distilled water or aqueous buffer of choice.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Contains Sucrose
Activity
>600 U/mg protein
One unit hydrolyses 1 µmole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.
For batch-specific activity, please inquire.
Approx. Protein Concentrations
Protein content is approximately 20-25% of total weight
Protein Molecular Weight
540 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt
P00722
GO Terms
GO:0005515 protein binding
GO:0004565 beta-galactosidase activity
GO:0030246 carbohydrate binding
GO:0046872 metal ion binding
GO:0005990 lactose catabolic process
GO:0009341 beta-galactosidase complex
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