- Product Form
- Purified AP from calf intestine - liquid
- Crystalline suspension. Free of endonuclease, exonuclease and RNase activities.
- Buffer Solution
- 70% Ammonium sulphate.
- Preservative Stabilisers
- None present
- 4000 U/mg protein.
1 unit liberates 1 µmole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-nitrophenyl phosphate.
- Approx. Protein Concentrations
- Total protein concentration 15.0 mg/ml
- Protein Molecular Weight
- 110-150 kDa
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
alkaline phosphatase activity
integral to membrane
metal ion binding
anchored to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Alkaline Phosphatase
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- The product may be diluted in sterile distilled water or in dilute buffer
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Alkaline Phosphatase
Winkelman, J. et al. (1972) The clinical usefulness of alkaline phosphatase isoenzyme determinations.
Am J Clin Pathol. 57 (5): 625-34.