Alkaline Phosphatase
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Product Details
- Product Form
- Purified AP from calf intestine - liquid
- Preparation
- Crystalline suspension. Free of endonuclease, exonuclease and RNase activities.
- Buffer Solution
- 70% Ammonium sulphate.
- Preservative Stabilisers
- None present
- Activity
- 4000 U/mg protein.
1 unit liberates 1 µmole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-nitrophenyl phosphate. - Approx. Protein Concentrations
- Total protein concentration 15.0 mg/ml
- Protein Molecular Weight
- 110-150 kDa
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19111
- Entrez Gene
- ALPI
- GO Terms
- GO:0005886 plasma membrane
- GO:0004035 alkaline phosphatase activity
- GO:0016021 integral to membrane
- GO:0046872 metal ion binding
- GO:0031225 anchored to membrane
- Regulatory
- For research purposes only
Applications of Alkaline Phosphatase
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- The product may be diluted in sterile distilled water or in dilute buffer
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Alkaline Phosphatase
-
Winkelman, J. et al. (1972) The clinical usefulness of alkaline phosphatase isoenzyme determinations.
Am J Clin Pathol. 57 (5): 625-34.
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