Alkaline Phosphatase

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Native Calf Alkaline Phosphatase

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0300-1706 E 5 mg

Product Details

Product Form
Purified AP from calf intestine - liquid
Preparation
Crystalline suspension. Free of endonuclease, exonuclease and RNase activities.
Buffer Solution
70% Ammonium sulphate.
Preservative Stabilisers
None present
Activity
4000 U/mg protein.
1 unit liberates 1 µmole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-nitrophenyl phosphate.
Approx. Protein Concentrations
Total protein concentration 15.0 mg/ml
Molecular Weight
MW: 110 - 150 kD

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P19111 Related reagents
Entrez Gene
ALPI Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004035 alkaline phosphatase activity
GO:0016021 integral to membrane
GO:0046872 metal ion binding
GO:0031225 anchored to membrane
Regulatory
For research purposes only

Applications of Alkaline Phosphatase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Instructions For Use
The product may be diluted in sterile distilled water or in dilute buffer.

Product Specific References

References for Alkaline Phosphatase

  1. Winkelman, J. et al. (1972) The clinical usefulness of alkaline phosphatase isoenzyme determinations.
    Am J Clin Pathol. 57 (5): 625-34.