CD56 antibody | rAB03-1H8

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Mouse anti Human CD56:StarBright Violet 515

Product Type: Monoclonal Antibody
Clone: rAB03-1H8
Isotype: IgG1
Specificity: CD56

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TZA062SBV515 Datasheet SDS SDS	F	100 Tests/0.5ml		Log in
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Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 1

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 2

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 3

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 4

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 5

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 6

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 7

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 8

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 9

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 thumbnail image 10

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 1 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD56 SBUV445 (**TZA062SBUV445**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 2 — Human PBMCs were stained with Anti-Human CD3 SBUV445 (**MCA6146SBUV445**) only (Figure A) or with Anti-Human CD3 SBUV445 (**MCA6146SBUV445**) and Anti-Human CD56 SBUV795 (**TZA062SBUV795**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 3 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD56 SBV440 (**TZA062SBV440**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 4 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD56 SBV515 (**TZA062SBV515**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 5 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD56 SBV610 (**TZA062SBV610**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 6 — Human PBMCs were stained with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) only (Figure A) or with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) and Anti-Human CD56 SBV790 (**TZA062SBV790**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 7 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD56 SBB580 (**TZA062SBB580**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 8 — Human PBMCs were stained with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) only (Figure A) or with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) and Anti-Human CD56 SBB675 (**TZA062SBB675**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 9 — Human PBMCs were stained with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) only (Figure A) or with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) and Anti-Human CD56 SBB765 (**TZA062SBB765**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Mouse Anti Human NCAM (CD56) Antibody, clone rAB03-1H8 gallery image 10 — Human PBMCs were stained with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) only (Figure A) or with Anti-Human CD3 SBUV400 (**(MCA6146SBUV400**) and Anti-Human CD56 SBY800 (**TZA062SBY800**) (Figure B). Experiments were performed on PBMCs gated on live, single cell lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

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Mouse anti Human CD56 antibody, clone rAB03-1H8, is a recombinant monoclonal antibody that recognizes CD56, also known as human neural cell adhesion molecule (NCAM).

CD56 is a cell surface glycoprotein of the immunoglobulin superfamily. In the human hematopoietic system, CD56 is expressed on natural killer (NK) cells, alpha beta T cells, gamma delta T cells, dendritic cells and monocytes. As the name suggests, it is also expressed by cells of neuronal lineage. Three main isoforms of CD56 exist that differ in the intracellular domain. In NK cells, CD56 has been shown to act as a pathogen recognition receptor, promote cytotoxicity and stimulate exocytosis. In neuronal tissues, CD56 mediates homophilic and heterophilic adhesion, and is implicated in neural development, cell migration and synaptic plasticity.

CD56 is considered a classical surface marker of NK cells, which are usually divided into two subsets, CD56^dim and CD56^bright. NK cells in peripheral blood are predominantly CD56^dim, whilst CD56^bright NK cells are more prevalent in tissues. Expression of CD56 by NK cells can be used as an activation marker. Aberrant CD56 expression is associated with a range of tumors and malignancies, including tumors of the lung, neural derived malignancies, and natural killer cell lymphomas.

Target Species: Human
Product Form: Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain and conjugated to StarBright Violet 515 – liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source: CHO cells
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: Recombinant CD56 Leu20-Ala708 expressed by mammalian cells
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Violet 515 402 516
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is covered by U.S Patent No. 10,150,841 and related U.S and foreign counterparts.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Synonyms: NCAM-1
UniProt: P13591
Entrez Gene: NCAM1