CD3 antibody | SK7
Mouse anti Human CD3:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- SK7
- Isotype
- IgG1
- Specificity
- CD3
Antibody Quality and Performance Guarantee
| Mouse anti Human CD3 antibody, clone SK7 recognizes the T-cell surface glycoprotein CD3 epsilon chain (CD3ɛ), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3ɛ is a subunit of the TCR-CD3 cell surface receptor complex, which is expressed at the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009). Deficiency of the CD3ε chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency (SCID) phenotype (Fischer et al. 2005). Mouse anti-human CD3, clone SK7 binding to CD3 has been shown to be blocked by clone OKT3 and 12F6 (Li et al. 2005). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Human CD3ɛ
- Purity
- >90 % by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 488 | ||||||
- UniProt
- P07766
- Entrez Gene
- CD3E
- GO Terms
- GO:0004888 transmembrane receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0007172 signal complex assembly
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0017124 SH3 domain binding
- GO:0019901 protein kinase binding
- GO:0030159 receptor signaling complex scaffold activity
- View More GO Terms
- GO:0031295 T cell costimulation
- GO:0042608 T cell receptor binding
- GO:0046982 protein heterodimerization activity
- GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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