CD45RC antibody | 3a56
Mouse anti Pig CD45RC:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 3a56
- Isotype
- IgG1
- Specificity
- CD45RC
Mouse anti Pig CD45RC, clone 3a56 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will detect the ~210 kDa RC isoform. CD45 in pigs has three cell surface expressed isoforms: CD45R, CD45RA, and CD45RC (Schnitzlein and Zuckermann 1998). CD45RC is mainly expressed by CD4+ T cells and weakly by B cells (Piriou-Guzylack and Salmon 2008). However, during the immune response expression of CD45RC was found to decrease in activated T-helper cells (Saalmuller et al. 2002). In contrast, increased expression of CD45RC was observed during the maturation of CD4- gamma delta thymocytes (Sinkora et al. 2005). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Further Reading
-
Zuckermann, F.A. et al. (2001) Characterization of monoclonal antibodies assigned to the CD45 subgroup of the Third International Swine CD Workshop.
Vet Immunol Immunopathol. 80 (1-2): 165-74. -
Piriou-Guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39 (6): 54. -
Schnitzlein, W.M. & Zuckermann, F.A. (1998) Determination of the specificity of CD45 and CD45R monoclonal antibodies through the use of transfected hamster cells producing individual porcine CD45 isoforms.
Vet Immunol Immunopathol. 60 (3-4): 389-401. -
Saalmüller, A. et al. (2002) T-helper cells from naive to committed.
Vet Immunol Immunopathol. 87 (3-4): 137-45. -
Sinkora, M. et al. (2005) Development of gammadelta thymocyte subsets during prenatal and postnatal ontogeny.
Immunology. 115 (4): 544-55.
MCA6049A647
1803If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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