CD221 antibody | 1H7
Mouse anti Human CD221:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 1H7
- Isotype
- IgG1
- Specificity
- CD221
Mouse anti Human CD221 antibody, clone 1H7 recognizes human CD221, a ~155 kDa receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by western blotting (Li et al.1993). CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signaling proteins. Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221 (Li et al.1993). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Purified human placental IGF-I receptor.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
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Description | Mouse IgG1 Negative Control:RPE |
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Human Seroblock | BUF070A | F | 50 Test | Log in | |||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
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Description | Human Seroblock |
References for CD221 antibody
-
Li, S.L. et al. (1993) Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
Biochem Biophys Res Commun. 196 (1): 92-8. -
Beauvais, D.M. & Rapraeger, A.C. (2010) Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation.
J Cell Sci. 123 (Pt 21): 3796-807. -
Krieger, C.C. et al. (2016) Thyrotropin/IGF-1 receptor crosstalk in Graves' ophthalmopathy pathogenesis.
J Clin Endocrinol Metab. Apr 4: jc20161315. [Epub ahead of print] -
van Valen, F. et al. (2012) A Novel Role of IGF1 in Apo2L/TRAIL-Mediated Apoptosis of Ewing Tumor Cells.
Sarcoma. 2012: 782970.
- Synonyms
- IGF-1 Receptor
- RRID
- AB_566646
- UniProt
- P08069
- Entrez Gene
- IGF1R
- GO Terms
- GO:0005010 insulin-like growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005158 insulin receptor binding
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005792 microsome
- GO:0006916 anti-apoptosis
- GO:0006955 immune response
- GO:0008284 positive regulation of cell proliferation
- View More GO Terms
- GO:0008286 insulin receptor signaling pathway
- GO:0042802 identical protein binding
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030335 positive regulation of cell migration
- GO:0031994 insulin-like growth factor I binding
- GO:0043548 phosphatidylinositol 3-kinase binding
- GO:0043559 insulin binding
- GO:0043560 insulin receptor substrate binding
- GO:0045740 positive regulation of DNA replication
- GO:0046777 protein autophosphorylation
- GO:0051262 protein tetramerization
MCA2344PE
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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