Browsing Modes for Business Users

IgA antibody | 15D6

ISO 9001 Quality Assurance Manufacturing | Print
Anti Human IgA Antibody, clone 15D6 thumbnail image 1 Anti Human IgA Antibody, clone 15D6 thumbnail image 2
Anti Human IgA Antibody, clone 15D6 gallery image 1
Human IgA detected with Mouse anti Human IgA:FITC (5104-3104F).
Anti Human IgA Antibody, clone 15D6 gallery image 2
Sandwich ELISA analysis of human IgA binding using Mouse anti Human IgA (MCA476G) as a capture reagent and biotinylated Mouse anti Human IgA (5104-3104) as a detection reagent with purified human IgA (PHP133) as antigen. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 25000 (Blue) and 50000 (Red) fold dilutions.

Mouse anti Human IgA:FITC

Product Type
Monoclonal Antibody
Clone
15D6
Isotype
IgG1
Specificity
IgA

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
5104-3104F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF 0.1 mg loader
List Price Your Price
loader

Mouse anti Human IgA antibody, clone 15D6 recognizes an epitope contained in the CH2 domain of the Fc fragment of Human IgA.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Purified Human IgA.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for IgA antibody

  1. Lorin, V. & Mouquet, H. (2015) Efficient generation of human IgA monoclonal antibodies.
    J Immunol Methods. 422: 102-10.

RRID
AB_1605048
UniProt
P01876
P01877
Entrez Gene
IGHA1
IGHA2
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0006955 immune response
GO:0018298 protein-chromophore linkage
GO:0005624 membrane fraction

5104-3104F

153723 160970

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with IgA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note