Sandwich ELISA analysis of human IgA binding using Mouse anti Human IgA (MCA476G) as a capture reagent and biotinylated Mouse anti Human IgA (5104-3104) as a detection reagent with purified human IgA (PHP133) as antigen. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 25000 (Blue) and 50000 (Red) fold dilutions
Purified Human Secretory IgA is derived from human colostrum and is predominately in dimeric form, the monomers being linked by immunoglobulin J chain.
Purified IgA - liquid
Purified human secretory IgA prepared by size-exclusion and ion exchange chromatography.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
2. Herrera, D. et al. (2013) Rotavirus specific plasma secretory immunoglobulin in children with acute gastroenteritis and children vaccinated with an attenuated human rotavirus vaccine.Hum Vaccin Immunother. 9 (11): 2409-17.