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Sandwich ELISA analysis of human IgA binding using Mouse anti Human IgA (MCA476G) as a capture reagent and biotinylated Mouse anti Human IgA (5104-3104) as a detection reagent with purified human IgA (PHP133) as antigen. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 25000 (Blue) and 50000 (Red) fold dilutions

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  • Purified Human Secretory IgA
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP133Edatasheet pdfdatasheet pdf0.5 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Purified Human Secretory IgA is derived from human colostrum and is predominately in dimeric form, the monomers being linked by immunoglobulin J chain.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgA - liquid
    • Reconstitution
    • Preparation
      Purified human secretory IgA prepared by size-exclusion and ion exchange chromatography.
    • Preservative Stabilisers
      None present
    • Purity
    • Approx. Protein Concentrations
      1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein-chromophore linkage
      immune response
      membrane fraction
      protein binding
      antigen binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgA Formats

    Formats Applications Sizes available
    IgA : Purified E 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

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