IgG antibody

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Goat anti Rat IgG:HRP (Mouse Adsorbed)

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.5 mg loader

Goat anti Rat IgG antibody recognizes rat IgG. Cross-reactivity with mouse immunoglobulins has been removed by solid phase adsorption.

Product Details

Target Species
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Rat IgG
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0003823 antigen binding
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000
Immunohistology - Frozen 1/50
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for IgG antibody

  1. Sato, J. et al. (2003) The fibrinolytic system in dissemination and matrix protein deposition during a mycobacterium infection.
    Am J Pathol. 163 (2): 517-31.
  2. Teupser, D. et al. (2004) Major reduction of atherosclerosis in fractalkine (CX3CL1)-deficient mice is at the brachiocephalic artery, not the aortic root.
    Proc Natl Acad Sci. 2004 Dec 21;101(51):17795-800
  3. Piletska, V.E. et al. (2014) Microplates with enhanced immobilization capabilities controlled by a magnetic field
    J Chin Adv Mat Soc. 2: 118-29.
  4. Thirunavukkarasu, S. et al. (2016) Cytochrome P450 1B1 Contributes to the Development of Angiotensin II-Induced Aortic Aneurysm in Male Apoe(-/-) Mice.
    Am J Pathol. 186 (8): 2204-19.
  5. Hadzhieva M et al. (2016) Relationship between natural and heme-mediated antibody polyreactivity.
    Biochem Biophys Res Commun. 472 (1): 281-6.
  6. VonFurstenberg, R.J. et al. (2011) Sorting mouse jejunal epithelial cells with CD24 yields a population with characteristics of intestinal stem cells.
    Am J Physiol Gastrointest Liver Physiol. 300 (3): G409-17.
  7. Mueller, M.A. et al. (2008) Prevention of atherosclerosis by the mTOR inhibitor everolimus in LDLR-/- mice despite severe hypercholesterolemia.
    Atherosclerosis. 198 (1): 39-48.
  8. Luo, Y.B. et al. (2013) Primary over-expression of AβPP in muscle does not lead to the development of inclusion body myositis in a new lineage of the MCK-AβPP transgenic mouse.
    Int J Exp Pathol. 94 (6): 418-25.
  9. Janardhan, K.S. et al. (2012) Integrin β3 is not critical for neutrophil recruitment in a mouse model of pneumococcal pneumonia.
    Cell Tissue Res. 348 (1): 177-87.
  10. Schmidt, N. et al. (2014) Dietary vitamin D inadequacy accelerates calcification and osteoblast-like cell formation in the vascular system of LDL receptor knockout and wild-type mice.
    J Nutr. 144 (5): 638-46.
  11. Harrison-findik, D.D. et al. (2009) Regulation of liver hepcidin expression by alcohol in vivo does not involve Kupffer cell activation or TNF-alpha signaling.
    Am J Physiol Gastrointest Liver Physiol. 296 (1): G112-8.
  12. Harcourt, L.J. et al. (2005) Interleukin-15 administration improves diaphragm muscle pathology and function in dystrophic mdx mice.
    Am J Pathol. 166 (4): 1131-41.
  13. Geoffrion, M. et al. (2014) Differential effects of glyoxalase 1 overexpression on diabetic atherosclerosis and renal dysfunction in streptozotocin-treated, apolipoprotein E-deficient mice.
    Physiol Rep. 2 (6)Jun 11 [Epub ahead of print].
  14. Elitok, S. et al. (2006) Cyclic arginine-glycine-aspartic acid peptide inhibits macrophage infiltration of the kidney and carotid artery lesions in apo-E-deficient mice.
    Am J Physiol Renal Physiol. 290 (1): F159-66.
  15. Pollow, J.D. et al. (2019) Menopause and FOXP3+ Treg cell depletion eliminate female protection against T cell-mediated angiotensin II hypertension.
    Am J Physiol Heart Circ Physiol. 317 (2): H415-H423.

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