IgM antibody

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Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR138A C E P WB 1 ml
Goat anti Mouse IgM antibody recognizes mouse IgM. Goat anti Mouse IgM antibody has been cross absorbed against mouse IgA, IgG1, IgG2a, IgG2b and IgG3, pooled human sera and purified human paraproteins. Goat anti Mouse IgM antibody shows minimal cross reactivity with human immunoglobulins.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to alkaline phosphatase - liquid
Antiserum Preparation
Antisera to mouse IgM were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on IgM.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Mouse IgM paraproteins.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01872 Related reagents
P01873 Related reagents
Entrez Gene
Ighm Related reagents
Ighm Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.