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IgG2c antibody

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Horseradish peroxidase conjugated Goat anti Mouse IgG2c antibody (STAR135P) used to evaluate IgG2c levels in mouse samples by ELISA.
Image caption:
rPmpD-specific IgG1 and IgG2c responses following immunization with different rPmpD vaccine formulations in C57BL/6 mice. Anti-rPmpD serum IgG1 and IgG2c titres were measured two weeks following penultimate and final immunisations (A, C) or six weeks post-challenge (B, D). Results are expressed as the geometric mean ± the standard deviation for groups of 5 mice measured in triplicate, and are representative of two separate experiments. (*P<0.05, **P<0.01).

From: Paes W, Brown N, Brzozowski AM, Coler R, Reed S, Carter D, Bland M, Kaye PM, Lacey CJ.
Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice. Vaccine. 2016 Jul 29;34(35):4123-31.

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Goat anti Mouse IgG2c:Alk. Phos.

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG2c

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STAR135A
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Goat anti Mouse IgG2c antibody recognizes mouse IgG2c, otherwise known as the Igh 1b allele of IgG2a. Goat anti Mouse IgG2c antibody is assessed by ELISA and immunoelectrophoresis and has been shown to react with IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice and does not react with L-chains or other IgG subclasses.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antisera to mouse IgG2c were raised by repeated immunisations of goats with highly purified antigen.
Buffer Solution
50mM HEPES, 0.1M Nacl, 1mM Mgcl2, 0.1mM Zncl2
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Native mouse IgG2c.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG2c antibody

  1. Jalili, R.B. et al. (2010) Local Expression of Indoleamine 2,3 Dioxygenase in Syngeneic Fibroblasts Significantly Prolongs Survival of an Engineered Three-Dimensional Islet Allograft.
    Diabetes 59: 2219-27
  2. Arnold, I.C. et al. (2011) The C-terminally encoded, MHC class II-restricted T cell antigenicity of the Helicobacter pylori virulence factor CagA promotes gastric preneoplasia.
    J Immunol. 186: 6165-72.
  3. Paes, W. et al. (2016) Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice.
    Vaccine. 34 (35): 4123-31.
  4. Carroll, E.C. et al. (2016) The Vaccine Adjuvant Chitosan Promotes Cellular Immunity via DNA Sensor cGAS-STING-Dependent Induction of Type I Interferons.
    Immunity. 44 (3): 597-608.
  5. Zouggari, Y. et al. (2013) B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction.
    Nat Med. 19 (10): 1273-80.
  6. Müller-Winkler, J. et al. (2021) Critical requirement for BCR, BAFF, and BAFFR in memory B cell survival.
    J Exp Med. 218 (2) [Epub ahead of print].
  7. McEntee, C.P. et al. (2020) Type I IFN signalling is required for cationic adjuvant formulation (CAF)01-induced cellular immunity and mucosal priming.
    Vaccine. 38 (3): 635-43.

Synonyms
Igh-1b Allele
RRID
AB_1102666
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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