IgG2c antibody
Horseradish peroxidase conjugated Goat anti Mouse IgG2c antibody (STAR135P) used to evaluate IgG2c levels in mouse samples by ELISA.
Image caption:
rPmpD-specific IgG1 and IgG2c responses following immunization with different rPmpD vaccine formulations in C57BL/6 mice. Anti-rPmpD serum IgG1 and IgG2c titres were measured two weeks following penultimate and final immunisations (A, C) or six weeks post-challenge (B, D). Results are expressed as the geometric mean ± the standard deviation for groups of 5 mice measured in triplicate, and are representative of two separate experiments. (*P<0.05, **P<0.01).
From: Paes W, Brown N, Brzozowski AM, Coler R, Reed S, Carter D, Bland M, Kaye PM, Lacey CJ.
Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice. Vaccine. 2016 Jul 29;34(35):4123-31.
Goat anti Mouse IgG2c antibody recognizes mouse IgG2c, otherwise known as the Igh 1b allele of IgG2a. Goat anti Mouse IgG2c antibody is assessed by ELISA and immunoelectrophoresis and has been shown to react with IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice and does not react with L-chains or other IgG subclasses.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antisera to mouse IgG2c were raised by repeated immunisations of goats with highly purified antigen.
- Buffer Solution
- 50mM HEPES, 0.1M Nacl, 1mM Mgcl2, 0.1mM Zncl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
0.05% Pro-CleanTM400 0.2% Bovine Serum Albumin - Immunogen
- Native mouse IgG2c.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG2c antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/10000 | |
| Flow Cytometry | |||
| ELISA | 1/10000 | 1/100000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 1x106 cells in 100ul.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG2c antibody
-
Jalili, R.B. et al. (2010) Local Expression of Indoleamine 2,3 Dioxygenase in Syngeneic Fibroblasts Significantly Prolongs Survival of an Engineered Three-Dimensional Islet Allograft.
Diabetes 59: 2219-27 -
Arnold, I.C. et al. (2011) The C-terminally encoded, MHC class II-restricted T cell antigenicity of the Helicobacter pylori virulence factor CagA promotes gastric preneoplasia.
J Immunol. 186: 6165-72. -
Paes, W. et al. (2016) Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice.
Vaccine. 34 (35): 4123-31. -
Carroll, E.C. et al. (2016) The Vaccine Adjuvant Chitosan Promotes Cellular Immunity via DNA Sensor cGAS-STING-Dependent Induction of Type I Interferons.
Immunity. 44 (3): 597-608. -
Zouggari, Y. et al. (2013) B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction.
Nat Med. 19 (10): 1273-80. -
Müller-Winkler, J. et al. (2021) Critical requirement for BCR, BAFF, and BAFFR in memory B cell survival.
J Exp Med. 218 (2) [Epub ahead of print]. -
McEntee, C.P. et al. (2020) Type I IFN signalling is required for cationic adjuvant formulation (CAF)01-induced cellular immunity and mucosal priming.
Vaccine. 38 (3): 635-43.
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