IgG antibody
alkaline phosphatase conjugated Goat anti Mouse IgG antibody (OBT1172) used to detect bound first antibody in western blotting for chi42 protein.
Image caption:
Analysis of western blot for chi42 genes were agroinfiltrated in N. benthamiana. (A):syncodChi42-1, (B): syncodChi42-2 and (C): chi42. M: Protein molecular weight marker (Thermo Scientific). P: purified CHI42 enzyme as positive control. N1 and N2: wild-type N. benthamiana and agroinfiltrated N. benthamiana without chi42 insert as nagative controls respectively. 1-3 after 3, 5 and 7 days of infiltration (pMYV719/chi42), 4-6 after 3, 5 and 7 days of coinfiltration (pMYV719/chi42 and pMYV508).
From: Tien NQD, PTB Hoa, NH Tue, DV Thanh, HA Thi, NN Luong, NX Huy, NH Loc (2021).
Transient expression of Chi42 genes from Trichoderma asperellum in Nicotiana benthamiana by agroinfiltration.
Intl J Agric Biol 26:177‒184
doi: 10.17957/IJAB/15.1822
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Alkaline prosphatase conjugated Goat anti Mouse IgG antibody (OBT1172) used to detect bound Mouse anti chitinase antibody by western blotting.
Image caption:
Western blot analysis of some chitinase transgenic peanut lines PC: bacterial puried chitinase 42 kDa as a positive control; NC: non-transgenic peanut as a negative control; 1-2 Page 14/18(Chi42): WTA-2 and WTA-4; 3-4-7 (syncodChi42-2): S2A-13, S2A-14 and S2A-12; 5-6 (syncodChi42-1): S1A-9 and S1A-15.
From: Res Sq 25 Mar 2022
doi: 10.21203/rs.3.rs-1487302/v1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetGoat anti Mouse IgG (multi-species Adsorbed):Alk. Phos.
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
| Goat anti Mouse IgG antibody recognises Mouse IgG. Cross-reactivity with Bovine, Chicken, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat or Sheep immunoglobulin has been minimised by solid phase absorption. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Antiserum Preparation
- Antiserum to mouse IgG was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on mouse IgG coupled agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
- Buffer Solution
- 0.05M TRIS Chloride
0.15M NaCl
0.001M MgCl2
0.0001M ZnCl2
50% (v/v) Glycerol; pH8.0 - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Mouse IgG whole molecule.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 | 1/10,000 |
| Immunohistology - Frozen | |
1/200 | 1/1000 |
| Western Blotting | |
1/500 | 1/2500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IgG antibody
-
Hoa, P.T.B. et al. (2022) Enhancement of resistance against fungal pathogens in peanut ( Arachis hypogaea. L.) cultivar L14 by heterologous expression of gene encoding chitinase 42 kDa from Trichoderma asperellum. SH16.
Res Sq. 25 Mar [Epub ahead of print]. -
Tien, N.Q et al. (2021) Transient Expression of Chi42 Genes from Trichoderma asperellum. in Nicotiana benthamiana. by Agroinfiltration.
Int J Agric Biol. 26 (01): 177-84.
- RRID
- AB_619827
- UniProt
- P01868
- P01869
- P01843
- P01845
- P01867
- P01865
- P01863
- P01864
- P03987
- P01844
- P01837
- Entrez Gene
- Igk-C
- LOC433053
- Iglc2
- Iglc3
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- View More GO Terms
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
OBT1172
151919
161615
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