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IgG antibody

Goat anti Mouse IgG (multi-species Adsorbed):Alk. Phos.

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

Product Code Applications Pack Size List Price Your Price Qty
OBT1172
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Goat anti Mouse IgG antibody recognises Mouse IgG. Cross-reactivity with Bovine, Chicken, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat or Sheep immunoglobulin has been minimised by solid phase absorption.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Antiserum Preparation
Antiserum to mouse IgG was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on mouse IgG coupled agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
Buffer Solution
0.05M TRIS Chloride
0.15M NaCl
0.001M MgCl2
0.0001M ZnCl2
50% (v/v) Glycerol; pH8.0
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Mouse IgG whole molecule.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/10,000
Immunohistology - Frozen 1/200 1/1000
Western Blotting 1/500 1/2500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG antibody

  1. Hoa, P.T.B. et al. (2022) Enhancement of resistance against fungal pathogens in peanut ( Arachis hypogaea. L.) cultivar L14 by heterologous expression of gene encoding chitinase 42 kDa from Trichoderma asperellum. SH16.
    Res Sq. 25 Mar [Epub ahead of print].
  2. Tien, N.Q et al. (2021) Transient Expression of Chi42 Genes from Trichoderma asperellum. in Nicotiana benthamiana. by Agroinfiltration.
    Int J Agric Biol. 26 (01): 177-84.

RRID
AB_619827
UniProt
P01868
P01869
P01843
P01845
P01867
P01865
P01863
P01864
P03987
P01844
P01837
Entrez Gene
Igk-C
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

OBT1172

151919 161615

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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