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IgG antibody

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Anti Mouse IgG (H/L) Antibody thumbnail image 1
Anti Mouse IgG (H/L) Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (5178-2504) used for the detection of bound anti actin antibody in western blotting.
Image caption:
Low oscillatory shear stress Arf6 heterozygosity decreases Arf6 expression.
(D) primary lung EC protein expression fo Arf6 and β-actin from WT and Arf6 HET mice,

From: Gogulamudi VR, Islam MT, Durrant JR, Adeyemo AO, Trott DW, Hyuhn MH, Zhu W, Donato AJ, Walker AE, Lesniewski LA.
Heterozygosity for ADP-ribosylation factor 6 suppresses the burden and severity of atherosclerosis.
PLoS One. 2023 May 10;18(5):e0285253.
doi: 10.1371/journal.pone.0285253.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Mouse IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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Product Code Applications Pack Size List Price Your Price Qty
5178-2504
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E WB 2 mg loader
List Price Your Price
loader

Goat anti Mouse IgG antibody recognizes both the heavy and light chains of mouse IgG.
In immunoelectrophoresis a single precipitin arc was observed against anti-peroxidase, anti-goat serum, mouse IgG and mouse serum.

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG was prepared from whole serum by immunoaffinity chromatography using Mouse IgG followed by solid phase adsorption to remove unwanted reactivities.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1% Bovine Serum Albumin
0.01% Gentamicin sulphate
Immunogen
Mouse IgG, whole molecule.
Approx. Protein Concentrations
2.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/40000 1/80000
Immunohistology - Frozen 1/500 1/2500
Western Blotting 1/2000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG antibody

  1. Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
    J. Immunol. Methods 47: 129-144.
  2. Donner, K.M. et al. (2012) Generalized glucocorticoid resistance caused by a novel two-nucleotide deletion in the hormone-binding domain of the glucocorticoid receptor gene NR3C1.
    Eur J Endocrinol. 168: K9-K18.
  3. Gogulamudi, V.R. et al. (2023) Heterozygosity for ADP-ribosylation factor 6 suppresses the burden and severity of atherosclerosis.
    PLoS One. 18 (5): e0285253.
  4. Kakhan, V.S. et al (2024) Combined irradiation by gamma-rays and carbon-12 nuclei caused hyperlocomotion and change in striatal metabolism of rats
    Life Sci Space Res. 24 Aug [Epub ahead of print]

RRID
AB_619899
UniProt
P01864
P01867
P01865
P01863
P01868
P01869
P03987
P01844
P01843
P01845
P01837
Entrez Gene
Igk-C
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

5178-2504

153089 157577 168217

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