IgG antibody

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Goat anti Mouse IgG (H/L):HRP

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
5178-2504	C E WB			2 mg

Goat anti Mouse IgG antibody recognizes both the heavy and light chains of mouse IgG.
In immunoelectrophoresis a single precipitin arc was observed against anti-peroxidase, anti-goat serum, mouse IgG and mouse serum.

Product Details

Target Species

Mouse

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised

Reconstitution

Use 1 ml of sterile DI water. For extended storage reconstitute product with 50% glycerol instead of water and aliquot and store at -20ºC.

Preparation

Purified IgG was prepared from whole serum by immunoaffinity chromatography using Mouse IgG followed by solid phase adsorption to remove unwanted reactivities.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

1% Bovine Serum Albumin

0.01% Gentamicin sulphate

Immunogen

Mouse IgG, whole molecule.

Approx. Protein Concentrations

2.0 mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P01864 Related reagents; P01867 Related reagents; P01865 Related reagents; P01863 Related reagents; P01868 Related reagents; P01869 Related reagents; P03987 Related reagents; P01844 Related reagents; P01843 Related reagents; P01845 Related reagents; P01837 Related reagents
Entrez Gene: Igk-C Related reagents; LOC433053 Related reagents; Iglc2 Related reagents; Iglc3 Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Ighg2b Related reagents; Ighg1 Related reagents; Ighg1 Related reagents; AI324046 Related reagents
GO Terms: GO:0003823 antigen binding; GO:0009897 external side of plasma membrane; GO:0019815 B cell receptor complex; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0019731 antibacterial humoral response; GO:0042571 immunoglobulin complex, circulating; GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0001788 antibody-dependent cellular cytotoxicity; GO:0001798 positive regulation of type IIa hypersensitivity; GO:0001812 positive regulation of type I hypersensitivity; GO:0006910 phagocytosis, recognition; GO:0006911 phagocytosis, engulfment; GO:0006958 complement activation, classical pathway; GO:0050766 positive regulation of phagocytosis
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/40000	1/80000
Immunohistology - Frozen	1/500	1/2500
Western Blotting	1/2000	1/10000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description	Product Code	Pack Size	Applications
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Product Specific References

References for IgG antibody

Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
J. Immunol. Methods 47: 129-144.
Donner, K.M. et al. (2012) Generalized glucocorticoid resistance caused by a novel two-nucleotide deletion in the hormone-binding domain of the glucocorticoid receptor gene NR3C1.
Eur J Endocrinol. 168: K9-K18.

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