IgG antibody

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Goat anti Mouse IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5178-2504 C E WB 2 mg
Goat anti Mouse IgG antibody recognizes both the heavy and light chains of mouse IgG.
In immunoelectrophoresis a single precipitin arc was observed against anti-peroxidase, anti-goat serum, mouse IgG and mouse serum.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Use 1 ml of sterile DI water. For extended storage reconstitute product with 50% glycerol instead of water and aliquot and store at -20ºC.
Preparation
Purified IgG was prepared from whole serum by immunoaffinity chromatography using Mouse IgG followed by solid phase adsorption to remove unwanted reactivities.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1% Bovine Serum Albumin
0.01% Gentamicin sulphate
Immunogen
Mouse IgG, whole molecule.
Approx. Protein Concentrations
2.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
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GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/40000 1/80000
Immunohistology - Frozen 1/500 1/2500
Western Blotting 1/2000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG antibody

  1. Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
    J. Immunol. Methods 47: 129-144.
  2. Donner, K.M. et al. (2012) Generalized glucocorticoid resistance caused by a novel two-nucleotide deletion in the hormone-binding domain of the glucocorticoid receptor gene NR3C1.
    Eur J Endocrinol. 168: K9-K18.
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