IgG antibody
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Goat anti Mouse IgG antibody recognizes both the heavy and light chains of mouse IgG.
In immunoelectrophoresis a single precipitin arc was observed against anti-peroxidase, anti-goat serum, mouse IgG and mouse serum.
In immunoelectrophoresis a single precipitin arc was observed against anti-peroxidase, anti-goat serum, mouse IgG and mouse serum.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
- Reconstitution
- Use 1 ml of sterile DI water. For extended storage reconstitute product with 50% glycerol instead of water and aliquot and store at -20ºC.
- Preparation
- Purified IgG was prepared from whole serum by immunoaffinity chromatography using Mouse IgG followed by solid phase adsorption to remove unwanted reactivities.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
1% Bovine Serum Albumin 0.01% Gentamicin sulphate - Immunogen
- Mouse IgG, whole molecule.
- Approx. Protein Concentrations
- 2.0 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01864
- P01867
- P01865
- P01863
- P01868
- P01869
- P03987
- P01844
- P01843
- P01845
- P01837
- Entrez Gene
- Igk-C
- LOC433053
- Iglc2
- Iglc3
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/40000 | 1/80000 | |
| Immunohistology - Frozen | 1/500 | 1/2500 | |
| Western Blotting | 1/2000 | 1/10000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for IgG antibody
-
Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
J. Immunol. Methods 47: 129-144. -
Donner, K.M. et al. (2012) Generalized glucocorticoid resistance caused by a novel two-nucleotide deletion in the hormone-binding domain of the glucocorticoid receptor gene NR3C1.
Eur J Endocrinol. 168: K9-K18.
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