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IgA antibody

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Goat anti Mouse IgA:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

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STAR137F
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Goat anti Mouse IgA antibody recognizes Mouse IgA and has been cross absorbed against mouse IgM, IgG1, IgG2a, IgG2b and IgG3.

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to mouse IgA were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse IgA paraproteins.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence Neat 1/10
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for IgA antibody

  1. Capoccia, B.J. et al. (2011) Transcription factor MIST1 in terminal differentiation of mouse and human plasma cells.
    Physiol Genomics. 43 (3): 174-86.
  2. Yamashita, H. et al (2012) Overcoming food allergy through acquired tolerance conferred by transfer of Tregs in a murine model.
    Allergy. 67: 201-9.
  3. Won, Y.S. et al. (2017) Green tea cultivar 'Benifuuki' potentiates split vaccine-induced immunoglobulin A production.
    J Nat Med. 71 (1): 68-75.
  4. Ramstead, A.G. et al. (2016) Roles of Toll-Like Receptor 2 (TLR2), TLR4, and MyD88 during Pulmonary Coxiella burnetii Infection.
    Infect Immun. 84 (4): 940-9.
  5. Sessevmez, M. et al. (2023) Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously
    J Drug Deliv Sci Technol. 86: 104704.
  6. Zhang, S. et al. (2023) Select symbionts drive high IgA levels in the mouse intestine.
    Cell Host Microbe. 31 (10): 1620-1638.e7.

RRID
AB_2075636
UniProt
P01878
Entrez Gene
Igh-VJ558
GO Terms
GO:0003823 antigen binding

STAR137F

148278 165122

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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