CD194 antibody

Rabbit F(ab')2 anti Mouse CD194

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD194

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AAM66
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Rabbit anti mouse CD194 antibody recognizes mouse CD194, otherwise known as CCR4, C-C chemokine receptor type 4, C-C CKR-4or Cmkbr4. CD194 is a 360 amino acid ~42 kDa transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by CD4+ Th2 (type 2 helper T) cells, which acts as a high affinity receptor for the chemokines MDC (macrophage-derived chemokine), MCP-1, MIP-1, RANTES and TARC.

Rabbit anti mouse CD194 polyclonal antibody is reported as suitable for use in immunocytochemistry on human peripheral blood leucocytes or Jurkat cells.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
F(ab')2 fragment of purified IgG - liquid
Antiserum Preparation
Antiserum to mouse CD194 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide DTTQDETVYNSYYFYESMP-C corresponding to amino acids 8-26 of mouse CD194.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50000
Flow Cytometry 1/20
Immunohistology - Frozen 1/300
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
This product is suitable for use in indirect ELISA applications.

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Synonyms
CCR4
RRID
AB_2074510
UniProt
P51680
Entrez Gene
Ccr4
GO Terms
GO:0001764 neuron migration
GO:0002507 tolerance induction
GO:0016021 integral to membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0009897 external side of plasma membrane
GO:0016493 C-C chemokine receptor activity
View more products with CD194 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products