CD194 antibody
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Rabbit anti mouse CD194 antibody recognizes mouse CD194, otherwise known as CCR4, C-C chemokine receptor type 4, C-C CKR-4or Cmkbr4. CD194 is a 360 amino acid ~42 kDa transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by CD4+ Th2 (type 2 helper T) cells, which acts as a high affinity receptor for the chemokines MDC (macrophage-derived chemokine), MCP-1, MIP-1, RANTES and TARC.
Rabbit anti mouse CD194 polyclonal antibody is reported as suitable for use in immunocytochemistry on human peripheral blood leucocytes or Jurkat cells.
Rabbit anti mouse CD194 polyclonal antibody is reported as suitable for use in immunocytochemistry on human peripheral blood leucocytes or Jurkat cells.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- F(ab')2 fragment of purified IgG - liquid
- Antiserum Preparation
- Antiserum to mouse CD194 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin - Immunogen
- Synthetic peptide DTTQDETVYNSYYFYESMP-C corresponding to amino acids 8-26 of mouse CD194.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P51680 Related reagents
- Entrez Gene
- Ccr4 Related reagents
- GO Terms
- GO:0001764 neuron migration
- GO:0002507 tolerance induction
- GO:0016021 integral to membrane
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0009897 external side of plasma membrane
- GO:0016493 C-C chemokine receptor activity
- Regulatory
- For research purposes only
Applications of CD194 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50000 | ||
| Flow Cytometry | 1/20 | ||
| Immunohistology - Frozen | 1/300 | ||
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- This product is suitable for use in indirect ELISA applications.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* | ||
| Histar Detection System | STAR3000A | 50 Tests | C P | ||
| Histar Detection System | STAR3000B | 150 Tests | C P | ||
| Histar Detection System | STAR3000C | 500 Tests | C P |
