CD194 antibody

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Rabbit F(ab')2 anti Mouse CD194

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM66 C E F WB 50 µl
Rabbit anti mouse CD194 antibody recognizes mouse CD194, otherwise known as CCR4, C-C chemokine receptor type 4, C-C CKR-4or Cmkbr4. CD194 is a 360 amino acid ~42 kDa transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by CD4+ Th2 (type 2 helper T) cells, which acts as a high affinity receptor for the chemokines MDC (macrophage-derived chemokine), MCP-1, MIP-1, RANTES and TARC.

Rabbit anti mouse CD194 polyclonal antibody is reported as suitable for use in immunocytochemistry on human peripheral blood leucocytes or Jurkat cells.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
F(ab')2 fragment of purified IgG - liquid
Antiserum Preparation
Antiserum to mouse CD194 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide DTTQDETVYNSYYFYESMP-C corresponding to amino acids 8-26 of mouse CD194.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P51680 Related reagents
Entrez Gene
Ccr4 Related reagents
GO Terms
GO:0001764 neuron migration
GO:0002507 tolerance induction
GO:0016021 integral to membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0009897 external side of plasma membrane
GO:0016493 C-C chemokine receptor activity
Regulatory
For research purposes only

Applications of CD194 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50000
Flow Cytometry 1/20
Immunohistology - Frozen 1/300
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
This product is suitable for use in indirect ELISA applications.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P