XIAP antibody

100% Secure


Rabbit anti XIAP

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00699
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human XIAP antibody recognizes the E3 ubiquitin-protein ligase XIAP, also known as XIAP, XLP2, BIRC5 or MIHA.

The XIAP gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein, BIRC5, functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. MIHA also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in XIAP are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of XIAP are found on chromosomes 2 and 11 (provided by RefSeq, Feb 2011).

Rabbit anti Human XIAP antibody detects a band of 53 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti XIAP detects a band of approximately 53 kDa in MOLT-4 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the C-terminal region of human XIAP

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P98170
Entrez Gene
XIAP
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0006916 anti-apoptosis
GO:0006974 response to DNA damage stimulus
GO:0008270 zinc ion binding
GO:0008629 induction of apoptosis by intracellular signals
GO:0016874 ligase activity
GO:0043027 caspase inhibitor activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of XIAP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml