XIAP antibody

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Anti XIAP Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti XIAP Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti XIAP Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti XIAP Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with XIAP antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 70 second exposure. Arrow points to XIAP (molecular weight 53 kDa).

Anti XIAP Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of XIAP knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti XIAP antibody (**VPA00699**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 130 second and (B) 30 second exposure.

Anti XIAP Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of XIAP from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Rabbit anti XIAP

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: XIAP

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00699 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human XIAP antibody recognizes the E3 ubiquitin-protein ligase XIAP, also known as XIAP, XLP2, BIRC5 or MIHA.

The XIAP gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein, BIRC5, functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. MIHA also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in XIAP are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of XIAP are found on chromosomes 2 and 11 (provided by RefSeq, Feb 2011).

Rabbit anti Human XIAP antibody detects a band of 53 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti XIAP detects a band of approximately 53 kDa in MOLT-4 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered glycine.
Preservative Stabilisers: 0.01% Thiomersal
<50% Glycerol
Immunogen: Recombinant protein encompassing part of the C-terminal region of human XIAP
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P98170
Entrez Gene: XIAP
GO Terms: GO:0005515 protein binding; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0006916 anti-apoptosis; GO:0006974 response to DNA damage stimulus; GO:0008270 zinc ion binding; GO:0008629 induction of apoptosis by intracellular signals; GO:0016874 ligase activity; GO:0043027 caspase inhibitor activity; View More GO Terms