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WNT-3 antibody

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Anti WNT-3 Antibody thumbnail image 1 Anti WNT-3 Antibody thumbnail image 2
Anti WNT-3 Antibody gallery image 1
Western blotting analysis of HEK293 cell line lysate (35μg protein in RIPA buffer) probed with Goat anti Human WNT-3 antibody (AHP2268) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Anti WNT-3 Antibody gallery image 2
Immunohistochemical staining of paraffin embedded Human Breast tissue sections probed with Goat anti mouse WNT-3 antibody (AHP2268) at 2.0μgml-1 following heat mediated antigen retrieval with citrate buffer pH 6.0

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Goat anti WNT-3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
WNT-3

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Product Code Applications Pack Size List Price Your Price Qty
AHP2268
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF P * WB 0.1 mg loader
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loader

Goat anti Wnt-3 antibody recognises both human and mouse proto-oncogene Wnt-3, a member of the Wnt family, and ligand of the frizzled family of seven transmembrane receptors.

Wnt-3, otherwise known as Int-4, is involved in cell fate decisions and patterning during embryogenesis, cell signalling during neural tube development, and implicated in the tumorogenesis of several cancers including breast, lung, hepatocellular(HCC), and gastric carcinoma.

Over-expression of Wnt-3 playes a pivotal role in the resistance of HER2-overexpressing breast tumors to the breast cancer treatment drug Trastuzumab, also known as Herceptin (Wu et al. 2012). The effects of Wnt-3 over-expression included the transactivated expression of EGFR (epidermal growth factor receptor) an increased expression of B-catenin within the nucleus, an increase in N-cadherin and the neural crest transcription factor SLUG, as well as promoting an EMT (epithelial to mesenchymal transition) response.

Goat anti Wnt-3 polyclonal antibody exhibits strong fibrous staining in myoepithelial cells of human breast tissue in paraffin sections.

Goat anti Wnt-3 polyclonal antibody stains mouse hippocampal primary astrocytes in ICC/IF (Okamoto et al. 2011).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Wnt-3 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-GRGHNTRTEKRKEK from the internal region of Wnt-3 (NP_110380.1).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1:16000
Immunofluorescence
Immunohistology - Paraffin 1 3.0 5.0ug/ml
Western Blotting 1.0 3.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast
Western Blotting
AHP2268 detects a band of approximately 40kDa in HEK293 cell lysates

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

Further Reading

  1. Wu, Y. et al. (2012) Expression of Wnt3 activates Wnt/β-catenin pathway and promotes EMT-like phenotype in trastuzumab-resistant HER2-overexpressing breast cancer cells.
    Mol Cancer Res. 10 (12): 1597-606.

Synonyms
INT4
UniProt
P56703
Entrez Gene
WNT3
GO Terms
GO:0005886 plasma membrane
GO:0000902 cell morphogenesis
GO:0001707 mesoderm formation
GO:0004871 signal transducer activity
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
GO:0005109 frizzled binding
GO:0005110 frizzled-2 binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005769 early endosome
GO:0005770 late endosome
GO:0009880 embryonic pattern specification
GO:0008284 positive regulation of cell proliferation
GO:0009950 dorsal/ventral axis specification
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030182 neuron differentiation
GO:0030917 midbrain-hindbrain boundary development
GO:0035115 embryonic forelimb morphogenesis
GO:0035116 embryonic hindlimb morphogenesis
GO:0042472 inner ear morphogenesis
GO:0044338 canonical Wnt receptor signaling pathway involved in mesenchymal stem cell differentiation
GO:0044339 canonical Wnt receptor signaling pathway involved in osteoblast differentiation
GO:0045121 membrane raft
GO:0045599 negative regulation of fat cell differentiation
GO:0060064 Spemann organizer formation at the anterior end of the primitive streak
GO:0060174 limb bud formation
GO:0060323 head morphogenesis
GO:0061180 mammary gland epithelium development
GO:0071300 cellular response to retinoic acid
GO:0071425 hemopoietic stem cell proliferation

AHP2268

153373 158644 163962

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