USP2 antibody

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Rabbit anti USP2

Rabbit anti-human USP2 antibody recognizes ubiquitin carboxyl-terminal hydrolase 2, also known as 41 kDa ubiquitin-specific protease, deubiquitinating enzyme 2, ubiquitin specific protease 12, ubiquitin specific protease 9 or ubiquitin thioesterase 2.

USP2 Antibody with Control Lysate

Rabbit anti-human USP2 antibody recognizes ubiquitin carboxyl-terminal hydrolase 2, also known as 41 kDa ubiquitin-specific protease, deubiquitinating enzyme 2, ubiquitin specific protease 12, ubiquitin specific protease 9 or ubiquitin thioesterase 2.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00475 WB 100 µl
VPA00475KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human USP2 antibody recognizes USP2 ealso known as ubiquitin carboxyl-terminal hydrolase 2, 41 kDa ubiquitin-specific protease, deubiquitinating enzyme 2, ubiquitin specific protease 12, ubiquitin specific protease 9, ubiquitin thioesterase 2 or ubiquitin-specific-processing protease 2.

The USP2 gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease required for TNF-alpha -induced NF-kB signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified (provided by RefSeq, Aug 2011).

Rabbit anti Human USP2 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti USP2 detects a band of approximately 80 kDa in HCT116 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the middle region of human USP2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition
400μg HCT116 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O75604 Related reagents
Entrez Gene
USP2 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007049 cell cycle
GO:0007517 muscle organ development
GO:0016579 protein deubiquitination
GO:0050821 protein stabilization
GO:0030332 cyclin binding
GO:0031625 ubiquitin protein ligase binding
GO:0045931 positive regulation of mitotic cell cycle
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only.

Applications of USP2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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