USP2 antibody

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Rabbit anti USP2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00475
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human USP2 antibody recognizes USP2 ealso known as ubiquitin carboxyl-terminal hydrolase 2, 41 kDa ubiquitin-specific protease, deubiquitinating enzyme 2, ubiquitin specific protease 12, ubiquitin specific protease 9, ubiquitin thioesterase 2 or ubiquitin-specific-processing protease 2.

The USP2 gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease required for TNF-alpha -induced NF-kB signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified (provided by RefSeq, Aug 2011).

Rabbit anti Human USP2 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti USP2 detects a band of approximately 80 kDa in HCT116 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the middle region of human USP2

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
O75604
Entrez Gene
USP2
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007049 cell cycle
GO:0007517 muscle organ development
GO:0016579 protein deubiquitination
GO:0050821 protein stabilization
GO:0030332 cyclin binding
GO:0031625 ubiquitin protein ligase binding
GO:0045931 positive regulation of mitotic cell cycle
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of USP2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml