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Troponin I antibody

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Goat anti Human Troponin I (Cardiac)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Troponin I
Region
(CARDIAC)

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Product Code Applications Pack Size List Price Your Price Qty
AHP1268
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF WB 0.2 mg
List Price Your Price

Goat anti Human Troponin I antibody recognizes human cardiac-specific troponin I, (TnI) an integral inhibitory protein of cardiac muscle which exists as a complex with troponin C (TnC) and troponin T (TnT).

The TnT subunit of troponin binds to tropomyosin to form a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, enabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement.

The measurement of blood TnI and TnT levels is an important diagnostic indicator of heart muscle damage, and can be used to differentiate between angina and myocardic infarction in patients with chest pain. Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by Immunoaffinity chromatography
Antiserum Preparation
Antisera to human cardiac troponin I were raised by repeated immunisations of goats with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Native human cardiac troponin I, purity greater than 98%.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for Troponin I antibody

  1. Jin, J. et al. (2009) Transplantation of mesenchymal stem cells within a poly(lactide-co-epsilon-caprolactone) scaffold improves cardiac function in a rat myocardial infarction model.
    Eur J Heart Fail. 11: 147-53.
  2. Demyanets, S. et al. (2013) Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature.
    J Mol Cell Cardiol. 60: 16-26.

Further Reading

  1. Takeda, S. (2005) Crystal structure of troponin and the molecular mechanism of muscle regulation.
    J Electron Microsc (Tokyo). 54 Suppl 1: i35-41.
  2. Jeremias, A. & Gibson, C.M. (2005) Narrative review: alternative causes for elevated cardiac troponin levels when acute coronary syndromes are excluded.
    Ann Intern Med. 142 (9): 786-91.

Immunofluorescence

Western Blotting

RRID
AB_844683
UniProt
P19429
Entrez Gene
TNNI3
GO Terms
GO:0001570 vasculogenesis
GO:0001980 regulation of systemic arterial blood pressure by ischemic conditions
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005861 troponin complex
GO:0006874 cellular calcium ion homeostasis
GO:0019855 calcium channel inhibitor activity
GO:0019901 protein kinase binding
GO:0019904 protein domain specific binding
GO:0030049 muscle filament sliding
GO:0030172 troponin C binding
GO:0031014 troponin T binding
GO:0032780 negative regulation of ATPase activity
GO:0048306 calcium-dependent protein binding
GO:0055010 ventricular cardiac muscle tissue morphogenesis
GO:0060048 cardiac muscle contraction

AHP1268

152747 160328 162236 162968 164511

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